Adjuvants for use in vaccines
View Patent ↗The invention relates to adjuvants that contain a lecithin, an oil and an amphiphilic surfactant and that are capable of forming a stable oil-in-water emulsion vaccine so as to minimize local reactions to the vaccine in the injected animal.
1. A vaccine comprising,
a) Bordetella brochiseptica cells that have been prepared from a culture of said cells where said cells have been inactivated by adding about 0.6% volume per volume concentration (“v/v”) of formalin followed by adding about 0.6% v/v of glutaraldehyde, where said cells are provided in an amount effective to protect a mammal against a Bordetella bronchiseptica infection, and
b) a physiologically acceptable carrier.
2. A vaccine comprising,
a) Bordetella bronchiseptica cells prepared from a culture of said cells, where said cells have been inactivated by adding about 0.60 a v/v of formalin followed by adding about 0.6% v/v of glutaraldehyde to said culture of said cells, where said cells are provided in an amount effective to protect a mammal against a Bordetella bronchiseptica infection,
b) an amount of toxigenic Pasteurella multocida antigens, where said antigens are provided in an amount effective to protect the mammal against a toxigenic Pasteurella multocida infection, and
c) a physiologically acceptable carrier.
3. A vaccine as in claim 1 or claim 2 , wherein the mammal is a pig.
4. A. method for protecting a pig against a Borderella bronchiseptica infection comprising vaccinating the pig with a vaccine comprising,
a) Bordetella brochiseptica cells that have been prepared from a culture of said cells where said cells have been inactivated by adding about 0.6% v/v of formalin followed by adding about 0.6% v/v of glutaraldehyde, where said cells are provided in an amount effective to protect the pig against a Bordetella bronchiseptica infection, and
b) a physiologically acceptable carrier.
5. A method for protecting a pig against a Bordetella bronchiseptica infection and toxigenic Pasteurella multocida infection comprising, vaccinating the pig with a vaccine comprising,
i) Bordetella bronchiseptica cells that have been prepared from a culture of said cells, where said cells have been inactivated by adding about 0.6% v/v of formalin followed by adding about 0.6% v/v of glutaraldehyde to said culture of said cells, where said cells are provided in an amount effective to protect the pig against a Bordetella bronchiseptica infection,
ii) an effective amount of Pasteurella multocida antigens, and
iii) a physiologically acceptable carrier.