IP Library › Granted Patent US 7,144,989
Granted Patent B2
US 7,144,989 · App. 10/957,200 · Granted Dec 5, 2006

Pegylated non-hypertensive hemoglobins, methods of preparing same, and uses thereof

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Quick Facts
Patent No.
US 7,144,989
App. No.
10/957,200
Granted
Dec 5, 2006
Kind
B2
Abstract

The present invention provides pegylated hemoglobins comprising a thiocarbamoyl-phenyl-polyethylene glycol (PEG) attached to hemoglobin, and comprising a polyethylene glycol (PEG) attached to hemoglobin by an acyl group. The invention also provides methods of preparing pegylated hemoglobins using isothiocyanato phenyl carbamate of PEG and using isothiocyanato phenyl di-PEG carbamate. The invention further provides compositions and blood substitutes comprising pegylated hemoglobins and methods of treating a subject which comprise administering to the subject blood substitutes comprising non-hypertensive pegylated hemoglobins.

Claims (13)

1. A PEGgylated hemoglobin comprising a thiocarbamoyl-phenyl-carbamate of polyethylene glycol (PEG) attached to hemoglobin.

2. The PEGylated hemoglobin of claim 1 , wherein the thiocarbamoyl-phenyl-polyethylene glycol (PEG) is attached to an α-amino group or to an ε-amino group of the hemoglobin.

3. The PEGylated hemoglobin of claim 1 , comprising a PEG with a molecular weight of 200–40,000 daltons.

4. The PEGylated hemoglobin of claim 1 , wherein the polyethylene glycol (PEG) has a molecular weight of 3,000–5,000 daltons.

5. The PEGylated hemoglobin of claim 1 , wherein the polyethylene glycol (PEG) has a molecular weight of 5,000 daltons.

6. The PEGylated hemoglobin of claim 1 , comprising the structure shown in the bottom of FIG. 1 or in the bottom of FIG. 17 .

7. The PEGylated hemoglobin of claim 1 , wherein two to eight thiocarbamoyl-phenyl-polyethylene glycol (PEG) groups are attached to the hemoglobin.

8. The PEGylated hemoglobin of claim 1 , wherein six thiocarbamoyl-phenyl-polyethylene glycol (PEG) groups are attached to the hemoglobin.

9. The PEGylated hemoglobin of claim 1 , having a radius of at least 5 nm.

10. The PEGylated hemoglobin of claim 1 , wherein the PEGylated hemoglobin has a viscosity that is at least 3 times greater than non-PEGylated hemoglobin.

11. The PEGylated hemoglobin of claim 1 , wherein the PEGylated hemoglobin has a colloidal osmotic pressure that is at least 4 times greater than non-PEGylated hemoglobin.

12. A composition comprising the PEGylated hemoglobin of claim 1 and a pharmaceutically acceptable carrier.

13. A blood substitute comprising the PEGylated hemoglobin of claim 1 .

Assignments (4)
CHANGE OF NAME Recorded Oct 20, 2015
From: COM AFFILIATION, INC.
To: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.
Reel/Frame 036907/0536 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 16, 2015
From: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY
To: COM AFFILIATION, INC.
Reel/Frame 036876/0363 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 2, 2005
From: SMITH, PAUL K.
To: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY
Reel/Frame 016647/0270 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 31, 2005
From: ACHARYA, SEETHARAMA A.; MANJULA, BELUR N.
To: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY
Reel/Frame 016416/0431 →