Card-domain containing polypeptides, encoding nucleic acids, and methods of use
View Patent ↗The invention provides caspase recruitment domain (CARD)-containing polypeptides and functional fragments thereof, encoding nucleic acid molecules, and specific antibodies. Also provided are screening methods for identifying CARD-associated polypeptides (CAPs), and for identifying agents that alter the association of a CARD-containing polypeptide with itself or with a CAP. Further provided are methods of altering a biochemical process modulated by a CARD-containing polypeptide, and methods of diagnosing a pathology characterized by an increased or decreased level of a CARD-containing polypeptide.
1. An isolated nucleic acid molecule consisting of a nucleic acid sequence encoding the CARD-12X polypeptide amino acid sequence SEQ ID NO:16.
2. The isolated nucleic acid molecule of claim 1 , said nucleic acid molecule consisting of the nucleotide sequence SEQ ID NO:15.
3. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule is cDNA or mRNA.
4. A vector containing the nucleic acid molecule of claim 1 .
5. An isolated host cell containing the nucleic acid molecule of claim 1 .
6. A method of producing a CARD-12X polypeptide, comprising expressing the nucleic acid molecule of claim 1 in vitro or in an isolated host cell under conditions suitable for expression of said polypeptide.
7. An isolated nucleic acid molecule consisting of a nucleic acid sequence encoding the CARD-12X CARD domain amino acid sequence SEQ ID NO:18.
8. The isolated nucleic acid molecule of claim 7 , said nucleic acid molecule consisting of the nucleotide sequence SEQ ID NO:17.
9. The nucleic acid molecule of claim 7 , wherein said nucleic acid molecule is cDNA or mRNA.
10. A vector containing the nucleic acid molecule of claim 7 .
11. An isolated host cell containing the nucleic acid molecule of claim 7 .
12. A method of producing a CARD-12X CARD domain polypeptide, comprising expressing the nucleic acid molecule of claim 7 in vitro or in an isolated host cell under conditions suitable for expression of said polypeptide.