Chromatography system, method and software for the separation of biomolecules
View Patent ↗The present invention relates to methods, systems and software for optimising the separation of a sample of biomolecules eluted from a chromatography column in which the concentration of a second component added to an elution buffer is varied in order to form an elution buffer solution of varying second component concentration. The concentration of the elution buffer solution is held at a current value when a hold gradient event occurs and the the varying of the concentration of the elution buffer solution is restarted when a restart gradient event occurs. A hold gradient event occurs when a detector signal relating to the amount of biomolecules in the elution buffer solution rises past a first threshold and a restart gradient event occurs when the signal falls past a second threshold.
1. A chromatography system for the separation of biomolecules, comprising a chromatography column containing a medium to which biomolecules can bind and means for eluting biomolecules from the column using an elution buffer solution, said elution buffer solution having a variable concentration of a second component, wherein the concentration of said second component is controllable by a control device containing software which can cause said concentration of said second component to change at a desired gradient and wherein said control device is able to receive output signals from a detector, is able to produce output signals related to amount of biomolecules in the elution buffer solution eluted from said column, wherein changes in the concentration of said second component in said elution buffer solution are prevented when said software detects a hold gradient event and further changes in said concentration are permitted when said software detects a restart gradient event.
2. The chromatography system of claim 1 , wherein a hold gradient event is considered to have occurred when said output signal from said detector is equal to or greater than a first threshold signal level (Bmin 1 ).
3. The chromatography system of claim 2 , wherein a restart gradient event is considered to have occurred when said output signal from said detector is less than a second threshold signal level (Bmin 2 ).
4. The chromatography system of claim 3 , wherein the value of the second threshold signal level Bmin 2 is not the same as the value of the first threshold signal level Bmin 1 .
5. The chromatography system of claim 1 , wherein a hold gradient event is considered to have occurred when said output signal from said detector changes at a rate which is equal to or greater than a first threshold signal level rate of change (Rmin 1 ).
6. The chromatography system of claim 1 , wherein a restart gradient event is considered to have occurred when said output signal from said detector changes at a rate which is less than a second threshold signal level rate of change (Bmin 2 ).