Functional surface coating
Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.
1. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group, wherein said functional group is an electrophilically active group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface.
2. The method of claim 1 , wherein said affixing further comprises coating said active component, said cross-linking component and said matrix forming component onto said substrate creating a coated substrate and curing said coated substrate.
3. The method of claim 2 , wherein said curing is thermal activation.
4. The method of claim 1 , wherein said functional group is selected from the group consisting of an amine-reactive group and a succinimide ester.
5. The method of claim 1 , wherein said spacer group is a hydrophilic polymer.
6. The method of claim 1 , wherein said spacer group comprises polyethylene glycol.
7. The method of claim 1 , wherein said spacer group comprises linear, star-shape, multi-arm, or comb-like polyethylene glycol.
8. The method of claim 1 , wherein said spacer group comprises linear polyethylene glycol.
9. The method of claim 1 , wherein said spacer group comprises multi-arm polyethylene glycol.
10. The method of claim 1 , wherein said binding group is a silane.
11. The method of claim 1 , wherein said binding group is an alkoxysilane or chlorosilane.
12. The method of claim 1 , wherein said binding group is an alkoxysilane.
13. The method of claim 1 , wherein said matrix-forming component is a polyoxyethylene-based surface-active substance.
14. The method of claim 1 , wherein said matrix-forming component is surface-active substance comprising polyoxyethylene sorbitan.
15. The method of claim 1 , wherein said matrix-forming component is surface-active substance comprising polyoxyethylene sorbitan tetraoleate.
16. The method of claim 1 , wherein said active component comprises an electrophilically active group, a hydrophilic polymer, and a silane.
17. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component; providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface, wherein said functional group is an amine-reactive or thiol-reactive group.
18. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface, wherein said cross-linking component is an organosilane or an azidosilane.
19. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface, wherein said active component comprises an amine-reactive group, a polyethylene glycol, and a silane.
20. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface, wherein said substrate is a solid surface.
21. A method of preparing a functional surface on a substrate, said method comprising:
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix-forming component onto said substrate, thereby forming a functional surface, wherein said substrate comprises a glass, a plastic, a semiconductor, or a metal.