Gel-shell beads with adsorbed or bound biomolecules
View Patent ↗Disclosed are gel-coated beads (including Hydrogel™-coated beads), which are capable of adsorbing, or absorbing, proteins and other biomolecules onto or into the gel coating. The gel-coated beads with absorbed or adsorbed biomolecules are suitable for use in an assays, purification or other purposes. The beads have a core made from any of a number of materials, including latex, coated with the gel shell. The biomolecules can be retained within the gel, following adsorption, by covalent attachment, or, by selection of conditions of ambient pH and/or ionic strength such that they are retained without further reaction. Therefore, adsorbed proteins would retain the ability to bind to their respective ligands.
1. A method of detecting particular binding molecules in a sample, comprising:
providing a set of encoded beads wherein, said beads having a core and a shell, where the shell is an ionic hydrogel composed of 6% hydroxyethyl methacrylate, 20% methacrylic acid and 74% methyl methacrylate by weight;
immobilizing, without covalent bonding, Protein L in the ionic hydrogel using PBS as a buffer where the pH is 3 such that said Protein L is absorbed and trapped in the ionic hydrogel;
suspending the encoded beads with immobilized Protein L therein, in a PBS buffer of pH 7.2 containing 0.1% BSA and 0.1% sodium azide by weight;
contacting the beads with said binding molecules; and
determining whether binding of said binding molecules to said Protein L takes place to thereby detect said particular binding molecules in the sample based on detection of signal from labeling associated with the binding event.
2. The method of claim 1 wherein the binding molecules are antibodies, antigens or enzymes.
3. The method of claim 2 , wherein the labeling is with a labeled secondary antibody, or another labeled molecule, which binds to the binding molecules.
4. The method of claim 1 wherein said beads are substantially the same size.
5. The method of claim 4 wherein beads have a coefficient of variation of their mean diameter of about five percent or less.