Culture medium with yeast or soy bean extract as amino acid source and no protein complexes of animal origin
The present invention relates to a medium containing non-animal derived proteinaceous material for cultivating patheogenic bacteria to produce an immunogenic factor and processes employing such medium.
1. A medium for cultivating Haemophilus influenzae to produce an immunogenic factor wherein the medium comprises a Haemophilus influenzae bacteria and at least about 50% by dry weight of a non-animal derived proteinaceous material, and which does not comprise animal derived proteinaceous material, wherein the non-animal derived proteinaceous material is a soybean derived protein composition.
2. A process for preparing an immunogenic factor of the Haemophilus influenzae bacteria comprising the steps of cultivating the Haemophilus influenzae bacteria in a medium comprising at least about 50% by dry weight of a non-animal derived proteinaceous material, wherein the medium does not comprise animal derived proteinaceous material and the non-animal derived proteinaceous material is a soybean derived protein composition.
3. The method of claim 2 , further comprising purifying the immunogenic factor from the medium.
4. A process for the production of a vaccine comprising preparing an immunogenic factor of Haemophilus influenzae by the process of claim 3 , and bringing said factor, optionally toxoided, into association with a pharmaceutically acceptable carrier.