Bioarray chip reaction apparatus and its manufacture
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
1 . A method of making probe chips comprising the steps of:
forming a plurality of probe arrays on a wafer;
separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
2 - 92 . (canceled)
93 . A septum penetrable by a member and which maintains a seal following member penetration in an axial direction and withdrawal, comprising: a first layer of resilient material having first and second opposed surfaces; and a second continuous layer extending across, and bonded to, the first surface of the first layer and which is in radial tension to hold the first layer in radial compression.
94 . A septum according to claim 93 additionally comprising a third layer extending across the second surface of the first layer, which third layer is in radial tension.
95 . A septum according to claim 94 wherein the third layer is continuous.
96 . A septum according to claim 94 wherein each of the second and third layers are under a tension of between 10 2 to 10 6 newton/m 2 .
97 . A septum according to claim 94 wherein each of the second and third layers comprise a resilient material.
98 . A septum according to claim 97 wherein each of the first, second and third layers comprise a resilient polymer.
99 . A septum according to claim 97 wherein each of the first, second and third layers has a thickness of less 10 mm.
100 . A septum according to claim 94 wherein the first layer is held in compression by the second and third layers.
101 . A septum according to claim 100 wherein the first layer is held in a compression of between 5 to 1000 newton/m.
102 . A septum assembly comprising a chamber having a rigid periphery defining an opening into the chamber, and a septum of claim 93 extended across, and supported by, the periphery.
103 . A septum assembly comprising a chamber having a rigid periphery defining an opening into the chamber, and a septum assembly of claim 94 extended across, and supported by, the periphery.
104 . A septum assembly of claim 103 wherein the opening has an area of 0.001 to 100 cm 2 .
105 . A septum assembly of claim 103 wherein the opening has an area of 0.01 cm 2 to 50 cm 2 .