Compositions and kits for detection of a nucleic acid by sequential amplification
View Patent ↗The invention relates to nucleic acid compositions for use in sequential amplification detection assays which permit the detection of a nucleic acid target in a sample.
1. A composition comprising:
(i) one or more template nucleic acids, each of which comprises in 3′ to 5′ order a first region, an extension region, and a second region;
(ii) a first oligonucleotide that is at least partially complementary to said first region of said template nucleic acid; and
(iii) one or more second oligonucleotides corresponding to said one or more template nucleic acids, each of said second oligonucleotides comprising a 5′ region and a 3′ region, wherein each said 3′ region is at least partially complementary to said second region of said corresponding template nucleic acid and wherein each said 5′ region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said first region of said corresponding template nucleic acid.
2. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region,
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region,
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid, and
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said extension region of said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid and said 5′ region is not complementary to said template nucleic acid.
3. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region,
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid, and
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is at least partially complementary to a region that may be upstream of, downstream of or comprising said first region of said target nucleic acid and is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to the second region of the template nucleic acid and the 5′ region is at least partially complementary to a region that may be upstream of, downstream of or comprising said first region of said template nucleic acid.
4. A composition comprising:
(i) one or more template nucleic acids, which comprise in 3′ to 5′ order a first region, a first extension region, a second region, a second extension region, and a third region;
(ii) an upstream primer that is at least partially complementary to said first region of said template nucleic acid;
(iii) one or more first downstream oligonucleotides corresponding to said one or more template nucleic acids, each said first downstream oligonucleotides comprising a 5′ region and a 3′ region, wherein each said 3′ region is at least partially complementary to said second region of said corresponding template nucleic acid and wherein each said 5′ region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said first region of said corresponding template nucleic acid;
(iv) one or more second downstream oligonucleotides corresponding to said one or more template nucleic acids, each said second downstream oligonucleotide comprising a 5′ region and a 3′ region, wherein each said 3′ region is at least partially complementary to said third region of said corresponding template nucleic acid and wherein said 5′ region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said second region of said corresponding template nucleic acid.
5. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region,
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, a first extension region, a second region, a second extension region and a third region,
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid, and
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said extension region of said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid, and wherein said 5′ region is not complementary to said first extension region of said template nucleic acid, and
(iv) a fourth oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said third region of said template nucleic acid, and wherein said 3′ region of said third oligonucleotide is not identical in sequence to said 3′ region of said fourth oligonucleotide, and wherein said 5′ region of said fourth oligonucleotide is not complementary to said second extension region of said template nucleic acid.
6. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region,
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, a first extension region, a second region, a second extension region and a third region,
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid, and
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is at least partially complementary to a region that may be upstream of, downstream of, or comprising said first region of said target nucleic acid and is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid, and wherein said 5′ region is at least partially complementary to a region that may be upstream of downstream of or comprising said first region of said template nucleic acid, and
(vi) a fourth oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said third region of said template nucleic acid, and wherein said 3′ region of said third oligonucleotide is not identical in sequence to said 3′ region of said fourth oligonucleotide, and wherein said 5′ region of said fourth oligonucleotide is at least partially complementary to a region that may be upstream of, downstream of or comprising said second region of said template nucleic acid.
7. The composition of any one of claims 1 to 6 further comprising a cleavage means which permits cleavage of a flap from a nucleic acid duplex structure and wherein said cleavage means lacks polymerase activity.
8. The composition of any one of claims 1 to 6 further comprising a nucleic acid polymerizing activity.
9. The composition of any one of claims 1 to 6 further comprising a cleavage means which permits cleavage of a flap from a nucleic acid duplex structure and which lacks polymerase activity and a nucleic acid polymerizing activity.
10. A kit comprising a composition of any one of claims 1 to 6 and packaging materials therefore.
11. The kit of claim 10 , further comprising a cleavage means which permits cleavage of a flap from a nucleic acid duplex structure and wherein said cleavage means lacks polymerase activity.
12. The kit of claim 10 , further comprising a nucleic acid polymerizing activity.
13. The kit of claim 10 , further comprising a cleavage means which permits cleavage of a flap from a nucleic acid duplex structure and which lacks polymerase activity and a nucleic acid polymerizing activity.
14. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region;
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region;
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid;
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid and said 5′ region is not complementary to said template nucleic acid.
15. A composition comprising:
(i) a target nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region;
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region, an extension region, and a second region;
(iii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid;
(iv) a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to the second region of the template nucleic acid and said 5′ region is at least partially complementary to a region that is 3′ of said second region of said template nucleic acid.
16. The composition of claim 14 or 15 , further comprising a cleavage means, wherein said cleavage means lacks polymerase activity.
17. The composition of claim 16 , wherein said cleavage means comprises a 5′ nuclease.
18. The composition of claim 14 or 15 , further comprising a polymerase.
19. The composition of claim 14 or 15 , further comprising a cleavage means and a polymerase.
20. The composition of claim 17 , wherein said 5′ nuclease is a FEN-1 nuclease.
21. The composition of claim 19 , wherein said polymerase and said cleavage means are contained in a single enzyme.
22. The composition of claim 19 , wherein said cleavage means is a FEN-1 nuclease and said polymerase is a Pfu DNA Polymerase.
23. The composition of claim 14 or 15 , wherein the 3′ nucleotide of said second oligonucleotide has a blocking group.
24. The composition of claim 14 or 15 , wherein the 3′ nucleotide of said template nucleic acid has a blocking group.
25. A kit comprising a composition of claim 14 or 15 and packaging materials therefore.
26. The kit of claim 25 , further comprising a cleavage means.
27. The kit of claim 25 , further comprising a nucleic acid polymerase.
28. The kit of claim 25 , further comprising a cleavage means and a nucleic acid polymerase.
29. The composition of claim 8 , wherein said polymerizing activity is a polymerase lacking 5′ to 3′ exonuclease activity.
30. The composition of claim 9 , wherein said polymerizing activity is a polymerase lacking 5′ to 3′ exonuclease activity.
31. The kit of claim 12 or 13 , wherein said polymerizing activity is a polymerase lacking 5′ to 3′ exonuclease activity.
32. The composition of claim 18 , wherein said polymerase lacks 5′ to 3′ exonuclease activity.
33. The composition of claim 19 , wherein said polymerase lacks 5′ to 3′ exonuclease activity.
34. The kit of claim 27 , wherein said nucleic acid polymerase lacks 5′ to 3′ exonuclease activity.
35. The kit of claim 28 , wherein said nucleic acid polymerase lacks 5′ to 3′ exonuclease activity.
36. The kit of claim 26 , wherein said cleavage means lacks polymerase activity.
37. The kit of claim 28 , wherein said cleavage means lacks polymerase activity.