The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis . The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.
1. An isolated polypeptide having cellulose 1,4-beta-cellobiosidase activity, selected from the group consisting of:
(a) a polypeptide having cellulose 1,4-beta-cellobiosidase activity comprising an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO: 5979; and
(b) a polypeptide having cellulose 1,4-beta-cellobiosidase activity encoded by a gene comprising a nucleotide sequence which hybridizes under high stringency conditions with the nucleotide sequence of SEQ ID NO: 1782, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
2. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1 , which comprises an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO: 5979.
3. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1 , which is encoded by a gene comprising a nucleotide sequence which hybridizes under high stringency conditions with the nucleotide sequence of SEQ ID NO: 1782, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% a formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% a SDS at 65° C.
4. The polypeptide having cellulose 2,4-beta-cellobiosidase activity of claim 1 , which is encoded by a Bacillus licheniformis ATCC 14580 gene.
5. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 2 , which comprises an amino acid sequence having at least 97% a identity with the amino acid sequence of SEQ ID NO: 5979.
6. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1 , which comprises the amino acid sequence of SEQ ID NO: 5979.
7. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1 , which consists of the amino acid sequence of SEQ ID NO: 5979, or a fragment thereof which retains cellulose 1,4-beta-cellobiosidase activity.