Methods and kits for the determination of sirolimus in a sample
View Patent ↗Methods and kits for determining sirolimus in a sample known or suspected to contain sirolimus are provided. The methods and kits according to the invention use one or more antibodies generated using a fragment of sirolimus, such as the northern fragment of sirolimus.
1. A method for determining sirolimus in a sample, comprising:
providing a sample known or suspected to contain sirolimus;
providing an antibody preparation comprising one or more antibodies generated using a compound consisting of the formula:
contacting the antibody preparation with the sample; and
detecting binding between the one or more antibodies and sirolimus.
2. The method for determining sirolimus in a sample according to claim 1 , wherein the sample comprises a human sample.
3. The method for determining sirolimus in a sample according to claim 1 , wherein the sample comprises a whole blood sample.
4. The method for determining sirolimus in a sample according to claim 1 , wherein the antibody preparation comprises a monoclonal antibody.
5. The method for determining sirolimus in a sample according to claim 4 , wherein the monoclonal antibody specifically binds whole sirolimus and does not specifically bind metabolized sirolimus.
6. The method for determining sirolimus in a sample according to claim 4 , wherein the monoclonal antibody is bound to a solid particle.
7. The method for determining sirolimus in a sample according to claim 1 , wherein the one or more antibodies specifically bind whole sirolimus and do not specifically bind metabolized sirolimus.
8. The method for determining sirolimus in a sample according to claim 1 , wherein the antibody preparation comprises one or more antibodies generated using a compound consisting of the formula:
bound to a carrier.
9. The method for determining sirolimus in a sample according to claim 8 , wherein the carrier comprises a protein.
10. The method for determining sirolimus in a sample according to claim 9 , wherein the carrier comprises one of ovalbumin, keyhole limpet hemocyanin, and hen eggwhite lysozyme.
11. The method for determining sirolimus in a sample according to claim 1 , further comprising the step of determining the concentration of sirolimus in the sample based upon the step of detecting binding between the one or more antibodies and sirolimus.
12. A method for determining a concentration of sirolimus in a sample, the method comprising:
contacting a sample known or suspected to contain sirolimus with an antibody preparation comprising one or more antibodies generated using a compound consisting of the formula:
determining the concentration of sirolimus in the sample by correlating the binding between the one or more antibodies and sirolimus in the sample to a series of known concentrations of sirolimus.
13. The method for determining a concentration of sirolimus in a sample according to claim 12 , wherein the sample comprises a human sample.
14. The method for determining a concentration of sirolimus in a sample according to claim 12 , wherein the sample comprises a whole blood sample.
15. The method for determining a concentration of sirolimus in a sample according to claim 12 , wherein the antibody preparation comprises a monoclonal antibody.
16. The method for determining a concentration of sirolimus in a sample according to claim 15 , wherein the monoclonal antibody specifically binds whole sirolimus and does not specifically bind metabolized sirolimus.
17. The method for determining a concentration of sirolimus in a sample according to claim 15 , wherein the monoclonal antibody is bound to a solid particle.
18. The method for determining a concentration of sirolimus in a sample according to claim 12 , wherein the one or more antibodies specifically bind whole sirolimus and do not specifically bind metabolized sirolimus.
19. The method for determining a concentration of sirolimus in a sample according to claim 12 , wherein the antibody preparation comprises one or more antibodies generated using the compound bound to a carrier.
20. The method for determining a concentration of sirolimus in a sample according to claim 19 , wherein the carrier comprises a protein.
21. The method for determining a concentration of sirolimus in a sample according to claim 20 , wherein the carrier comprises one of ovalbumin, keyhole limpet hemocyanin, and hen eggwhite lysozyme.