High-density amine-functionalized surface
A method for providing amine functional groups on a surface for binding proteins, peptides, DNAs, cells, small molecules, and other chemical or biological molecules that are of interests in the areas of proteomic, genomic, pharmaceutical, drug discovery, and diagnostic studies.
1 . A method for preparing a high-density amine-functionalized surface, comprising:
(a) treating a surface with epoxy silane to form an epoxy-functional surface; and
(b) attaching one or more amine-containing polymers to the epoxy-functional surface by adding a solution comprising one or more amine-containing polymers to the epoxy-functional surface under conditions where one or more amine-containing polymers react with the epoxy-functional surface; whereby a high-density amine-functionalized surface is formed.
2 . The method of claim 1 , further comprising the step of covalently attaching one or more chemical or biological molecules to the one or more amine-containing polymers attached to the surface.
3 . A biosensor comprising the high-density amine-functionalized surface.
4 . The biosensor of claim 3 , wherein the biosensor is an optical sensor.
5 . The biosensor of claim 4 , wherein the biosensor is a calorimetric resonant biosensor.
6 . The biosensor of claim 3 , wherein the biosensor is an acoustic biosensor.
7 . The biosensor of claim 3 , wherein the biosensor is an electric biosensor.
8 . The method of claim 1 , wherein the one or more amine-containing polymers are the same or different.
9 . The method of claim 1 , wherein the one or more amine-containing polymers are the same.
10 . The method of claim 1 , wherein the one or more amine-containing polymers comprise primary amines.
11 . The method of claim 1 , wherein the one or more amine-containing polymers comprise secondary amines.
12 . The method of claim 1 , wherein the one or more amine-containing polymers comprise primary and secondary amines.
13 . The method of claim 1 , wherein the one or more amine-containing polymers comprise polyethylenimine.
14 . The method of claim 1 , wherein the one or more amine-containing polymers consist of polyethylenimine.
15 . The method of claim 1 , wherein the one or more amine-containing polymers comprise polyvinylamine.
16 . The method of claim 1 , wherein the one or more amine-containing polymers consist of polyvinylamine.
17 . The method of claim 2 , wherein the chemical or biological molecules are selected from the group consisting of proteins, peptides, polypeptides, nucleotides, polynucleotides, small molecules, biotin, cells, fractionated cells, cells extracts, cell fractions, and parts of cells.
18 . The method of claim 17 , wherein the chemical or biological molecules comprise a protein.
19 . The method of claim 18 , wherein the protein is an enzyme.
20 . The method of claim 18 , wherein the protein is an antibody.
21 . The method of claim 18 , wherein the protein is selected from the group consisting of avidin or streptavidin.
22 . The method of claim 2 , wherein the chemical or biological molecules comprise a peptide.
23 . The method of claim 2 , wherein the chemical or biological molecules comprise a polynucleotide.
24 . The method of claim 2 , wherein the chemical or biological molecules comprise a cell, fractionated cell, cells extract, cell fraction, or part of a cell.
25 . The method of claim 2 , wherein the chemical or biological molecules comprise a small molecule.
26 . The method of claim 2 , wherein the chemical or biological molecules comprise biotin.
27 . A high-density amine-functionalized polymeric matrix, comprising one or more amine-containing polymers covalently attached to a surface through a functional epoxy, wherein the amine-containing polymers are the same or different, and wherein the amine-containing polymers comprise two or more amine groups.
28 . The high-density amine-functionalized polymeric matrix of claim 27 , wherein the amine-containing polymers comprise three or more amine groups.
29 . The high-density amine-functionalized polymeric matrix of claim 27 , wherein the amine-containing polymers comprise polyethylenimine.
30 . The high-density amine-functionalized polymeric matrix of claim 27 , wherein the amine-containing polymers comprise polyvinylamine.
31 . A method of immobilizing biomolecules on a surface, comprising contacting biomolecules with the high-density amine-functionalized surface of claim 1 , whereby the biomolecules are immobilized.
32 . A method of immobilizing biomolecules on a surface, comprising contacting biomolecules with the high-density amine-functionalized surface of claim 5 , whereby the biomolecules are immobilized.
33 . A method of immobilizing biomolecules on a surface, comprising contacting biomolecules with the high-density amine-functionalized surface of claim 13 , whereby the biomolecules are immobilized.
34 . A method of immobilizing biomolecules on a surface, comprising contacting biomolecules with the high-density amine-functionalized surface of claim 14 , whereby the biomolecules are immobilized.
35 . A biosensor comprising a high-density amine-functionalized surface, wherein the high-density amine-functionalized surface is prepared by the method comprising:
(a) treating a surface with epoxy silane to form an epoxy-functional surface; and
(b) attaching one or more amine-containing polymers to the epoxy-functional surface by adding a solution comprising one or more amine-containing polymers to the epoxy-functional surface under conditions where one or more amine-containing polymers react with the epoxy-functional surface; whereby a high-density amine-functionalized surface is formed.
36 . The biosensor of claim 35 , wherein the biosensor is an optical sensor.
37 . The biosensor of claim 36 , wherein the biosensor is a calorimetric resonant biosensor.
38 . The biosensor of claim 35 , wherein the biosensor is an acoustic biosensor.
39 . The biosensor of claim 35 , wherein the biosensor is an electric biosensor.
40 . The method of claim 1 , wherein the surface is a plastic surface.
41 . The method of claim 40 , further comprising the step of covalently attaching one or more chemical or biological molecules to the one or more amine-containing polymers attached to the surface.
42 . The biosensor of claim 3 , wherein the surface is a plastic surface.
43 . The biosensor of claim 35 , wherein the surface is a plastic surface.