IP Library › Patent Application 10984127
Patent Application
App. No. 10/984,127

Nucleic acid affinity columns

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Quick Facts
Patent No.
US None
App. No.
10/984,127
Abstract

This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided.

Claims (7)

1 . A method of making a nucleic acid pool comprising a plurality of different nucleic acids, said method comprising the steps of:

a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 20 oligonucleotides having different predetermined nucleic acid sequences, and wherein:

i) each different oligonucleotide is localized in a predetermined region of said surface;

ii) the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2; and

iii) said different oligonucleotides have an identical terminal 5′ nucleic acid sequence;

b) amplifying said multiplicity of oligonucleotides at least about 10 fold to provide said nucleic acid pool.

2 - 40 . (canceled)