Method of assessing skin and overall health of an individual
View Patent ↗The present invention relates to a method of determining skin health of an area of skin and overall health of an individual by exposing the area of skin to a first exposure radiation to induce the area of skin to emit a first fluorescent emission, measuring the intensity of the first fluorescent emission, exposing the area of skin to a second exposure radiation to induce the area of skin to emit a second fluorescent emission, measuring the intensity of the second fluorescent emission, calculating a ratio of these intensities, and comparing the ratio to a control ratio.
1. A method of assessing skin health, effects of aging, photoaging, or diabetic condition of a subject in question, said method comprising:
a. creating a control ratio by
(I) exposing a first area of skin not readily exposed to UV radiation of the subject in question to a first exposure radiation to induce said first area of skin to emit a first fluorescent emission, wherein said first exposure radiation comprises primarily of wavelengths of from about 290 nm to about 300 nm;
(II) measuring the intensity of said first fluorescent emission having a wavelength of from about 320 nm to about 350 nm to determine the amount of tryptophan in the skin;
(III) exposing said first area of skin to a second exposure radiation to induce said first area of skin to emit a second fluorescent emission, wherein said second exposure radiation comprises primarily of wavelengths of from about 330 nm to about 420 nm;
(IV) measuring the intensity of said second fluorescent emission having a wavelength of from about 380 nm to about 470 nm to determine the amount of collagen cross-links in the skin;
(V) calculating a ratio of said intensity measured in step (II) to said intensity measured in step (IV) to form a control ratio that cancels the effect of pigmentation to get a first tryptophan fluorescence parameter related to health and independent of pigmentation;
b. performing the measurements of steps (I), (II), (III) and (IV) for the subject in question on a second area of skin readily exposed to UV radiation;
c. calculating a ratio of the intensity measurements of step (II) of step b to the intensity measurement of step (IV) of step b for the subject in question to form a test ratio that cancels the effect of pigmentation to get a second tryptophan fluorescence parameter related to health and independent of pigmentation; and
d. comparing said test ratio to said control ratio to determine the skin health, effects of aging, photoaging, or diabetic condition of the subject in question.
2. A method of assessing skin health, effects of aging, photoaging, or diabetic condition of a subject in question, said method comprising:
a. creating a standard curve for a plurality of healthy individuals by, for each healthy individual:
(I) exposing an area of skin of each healthy individual to a first exposure radiation to induce said area of skin to emit a first fluorescent emission, wherein said first exposure radiation comprises primarily of wavelengths of from about 290 nm to about 300 nm;
(II) measuring the intensity of said first fluorescent emission having a wavelength of from about 320 nm to about 350 nm to determine the amount of tryptophan in the skin;
(III) exposing said area of skin to a second exposure radiation to induce said area of skin to emit a second fluorescent emission, wherein said second exposure radiation comprises primarily of wavelengths of from about 330 nm to about 420 nm;
(IV) measuring the intensity of said second fluorescent emission having a wavelength of from about 380 nm to about 470 nm to determine the amount of collagen cross-links in the skin;
(V) calculating a ratio of said intensity measured in step (II) to said intensity measured in step (IV) to cancel the effect of pigmentation to get a tryptophan fluorescence parameter related to health and independent of pigmentation;
(VI) plotting a standard curve for age of healthy individual versus the ratio of step (V);
b. performing the measurements of steps (I), (II), (III) and (IV) for a subject in question who is not a member of the plurality of healthy individuals in (a); and
c. calculating a ratio of said intensity measured in step (II) to said intensity measured in step (IV) for the subject in question to cancel the effect of pigmentation to get a tryptophan fluorescence parameter related to health and independent of pigmentation; and
d. comparing the ratio for the subject in question to the ratio plotted in the standard curve to determine the skin health, effects of aging, photoaging, or diabetic condition of the subject in question.
3. The method of claim 2 , wherein said first exposure radiation comprises primarily of wavelengths of about 295 nm.
4. The method of claim 3 , wherein said step (II) comprises measuring the intensity of said first fluorescent emission having a wavelength of about 340 nm.
5. The method of claim 4 , wherein said second exposure radiation comprises primarily of wavelengths of from about 390 nm to about 410 nm.
6. The method of claim 5 , wherein said step (IV) comprises measuring the intensity of said second fluorescent emission having a wavelength of about 440 nm.
7. The method of claim 3 , wherein said second exposure radiation comprises primarily of wavelengths of from about 390 nm to about 410 nm.
8. The method of claim 7 , wherein said step (IV) comprises measuring the intensity of said second fluorescent emission having a wavelength of about 440 nm.
9. The method of claim 2 , wherein said second exposure radiation comprises primarily of wavelengths of from about 390 nm to about 410 nm.
10. The method of claim 9 , wherein said step (IV) comprises measuring the intensity of said second fluorescent emission having a wavelength of about 440 nm.