Reduction of non-specific binding in assays
View Patent ↗Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.
1. A method for determining the presence and/or amount of an analyte in a sample suspected of containing said analyte, said method comprising:
(a) providing in combination said sample, a soluble compound comprising a protein linked to a first polysaccharide, and reagents for detecting said analyte wherein at least one of said reagents comprises a solid support comprising a second polysaccharide wherein said reagents comprise a specific binding partner for said analyte and wherein said protein does not bind to any of said reagents or to said analvte and wherein said first polysaccharide and said second polysaccharide may be the same or different,
(b) incubating said combination under conditions for binding of said analyte to one or more of said reagents, and
(c) detecting the presence and/or amount of binding of said analyte to said specific binding partner for said analyte, the presence and/or amount of said binding being related to the presence and/or amount of said analyte in said sample.
2. The method according to claim 1 wherein said soluble compound has a neutral charge or a negative charge.
3. The method according to claim 1 wherein said solid support comprises said specific binding partner for said analyte.
4. The method according to claim 3 wherein said at least one other of said reagents comprises a solid support comprising a second specific binding partner for said analyte.
5. The method according to claim 1 wherein said first polysaccharide and said second polysaccharide are the same.
6. The method according to claim 1 wherein said sample and said soluble compound are mixed together prior to forming said combination.
7. The method according to claim 1 wherein said soluble compound and said solid support are mixed together prior to forming said combination.
8. The method according to claim 1 wherein said first polysaccharide or said second polysaccharide is dextran or a dextran derivative.
9. The method according to claim 1 wherein said protein is a serum protein.
10. The method according to claim 9 wherein said serum protein is an albumin or a gamma globulin.
11. The method according to claim 1 wherein said solid support comprises particles.
12. The method according to claim 1 wherein said second polysaccharide is linked to said solid support and a member of a specific binding pair is linked to said second polysaccharide.
13. A method for determining the presence and/or amount of an analyte in a sample suspected of containing said analyte, said method comprising:
(a) providing in combination said sample, a soluble compound comprising a protein linked to a dextran or a dextran derivative, and reagents for detecting said analyte wherein at least one of said reagents comprises a solid support comprising a polysaccharide and a specific binding partner for said analyte, wherein said protein does not bind to any of said reagents or to said analyte,
(b) incubating said combination under conditions for binding of said analyte to said specific binding partner for said analyte, and
(c) detecting the presence and/or amount of binding of said analyte to said specific binding partner for said analyte, the presence and/or amount of said binding being related to the presence and/or amount of said analyte in said sample.
14. The method according to claim 13 wherein said polysaccharide is a dextran or a dextran derivative.
15. The method according to claim 13 wherein said reagents comprise a second specific binding partner for said analyte.
16. The method according to claim 13 wherein said protein is selected from the group consisting of gamma globulins, albumins, casein and gelatins.
17. The method according to claim 13 wherein said sample and said soluble compound are mixed together prior to forming said combination.
18. The method according to claim 13 wherein said soluble compound and said solid support are mixed together prior to forming said combination.
19. The method according to claim 13 wherein said protein is a an albumin.
20. The method according to claim 13 wherein said solid support comprises particles.
21. The method according to claim 13 wherein said polysaccharide is linked to said solid support and said specific binding partner for said analyte is linked to said polysaccharide.