IP Library › Granted Patent US 7,098,298
Granted Patent B2
US 7,098,298 · App. 10/992,133 · Granted Aug 29, 2006

Method for producing polyhydroxyalkanoate crystal

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Quick Facts
Patent No.
US 7,098,298
App. No.
10/992,133
Granted
Aug 29, 2006
Kind
B2
Abstract

The present invention provides a method for conveniently obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal comprises precipitating a polyhydroxyalkanoate crystal using a monohydric alcohol having 4 to 10 carbon atoms as a extraction solvent, keeping a polyhydroxyalkanoate solution containing 0.1 to 10% by weight of water relative to the total amount of the solution warm at 70° C. or higher, and cooling the solution to below 70° C.

Claims (19)

1. A method for producing a polyhydroxyalkanoate crystal which comprises extracting a polyhydroxyalkanoate using a monohydric alcohol having 4 to 10 carbon atoms as an extraction solvent, keeping a polyhydroxyalkanoate solution containing 0.1 to 10% by weight of water relative to the total amount of the solution warm at 70° C. or higher, and cooling the solution to below 70° C. to precipitate a polyhydroxyalkanoate crystal.

2. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the monohydric alcohol is selected from among butanol, pentanol, hexanol, heptanol, octanol, nonanol, decanol, and isomers thereof.

3. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate concentration in the polyhydroxyalkanoate solution is 1 to 20% by weight.

4. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate is a copolymer obtained by copolymerizing at least two species of monomers selected from the group consisting of 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxypropionate, 4-hydroxybutyrate, 4-hydroxyvalerate, 5-hydroxyvalerate, 3-hydroxyhexanoate, 3-hydroxyheptanoate, 3-hydroxyoctanoate, 3-hydroxynonanoate and 3-hydroxydecanoate.

5. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate is a copolymer composed of 3-hydroxyhexanoate and at least one species of hydroxyalkanoates other than 3-hydroxyhexanoate.

6. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate is a copolymer composed of 3-hydroxyhexanoate and 3-hydroxybutyrate.

7. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate is produced by at least one microorganism selected from the group consisting of species belonging to the genus Aeromonas, Alcaligenes, Azotobacter, Bacillus, Clostridium, Halobacterium, Nocardia, Rhodospirillum, Pseudomonas, Ralstonia, Zoogloea, Candida, Yarrowia, and Saccharomyces.

8. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

wherein the polyhydroxyalkanoate is produced by a transformant obtained by introducing a polyhydroxyalkanoate synthetic gene group derived from Aeromonas caviae.

9. The method for producing a polyhydroxyalkanoate crystal according to claim 8 ,

wherein the transformant obtained by introducing a polyhydroxyalkanoate synthetic gene group derived from Aeromonas caviae is Ralstonia eutropha obtained by introducing a polyhydroxyalkanoate synthetic gene group derived from Aeromonas caviae.

10. The method for producing a polyhydroxyalkanoate crystal according to claim 1 ,

which comprises extracting a polyhydroxyalkanoate, and using the remaining biomass substance after decreasing its solvent content as animal feed.

Assignments (11)
SECURITY INTEREST Recorded Dec 17, 2024
From: DANIMER SCIENTIFIC, INC.; MEREDIAN HOLDINGS GROUP, INC.; DANIMER SCIENTIFIC HOLDINGS, LLC; MEREDIAN, INC.; DANIMER SCIENTIFIC, L.L.C.; DANIMER BIOPLASTICS, INC.; DANIMER IPCO, LLC; DANIMER IPHOLDCO, LLC; NOVOMER, INC.
To: JEFFERIES CAPITAL SERVICES, LLC; RIVA RIDGE MASTER FUND, LTD.; BPI CREDIT 6, L.L.C.
Reel/Frame 069888/0715 →
SECURITY INTEREST Recorded Mar 22, 2023
From: DANIMER SCIENTIFIC, INC.; MEREDIAN HOLDINGS GROUP, INC.; DANIMER SCIENTIFIC HOLDINGS, LLC; DANIMER SCIENTIFIC MANUFACTURING, INC.; MEREDIAN, INC.; DANIMER SCIENTIFIC, L.L.C.; DANIMER SCIENTIFIC KENTUCKY, INC.; DANIMER BIOPLASTICS, INC.; DANIMER IPCO, LLC; NOVOMER, INC.; DANIMER IPHOLDCO, LLC
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS COLLATERAL AGENT
Reel/Frame 063146/0091 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 17, 2023
From: MEREDIAN, INC.
To: DANIMER IPCO, LLC
Reel/Frame 063113/0383 →
RELEASE OF SECURITY INTEREST Recorded Mar 16, 2023
From: SOUTHEAST COMMUNITY DEVELOPMENT FUND X, L.L.C.
To: DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.; DANIMER SCIENTIFIC, L.L.C.
Reel/Frame 063006/0860 →
RELEASE OF SECURITY INTEREST Recorded Jan 20, 2023
From: WHITE OAK GLOBAL ADVISORS, LLC
To: DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.,; DANIMER SCIENTIFIC, L.L.C.
Reel/Frame 062440/0763 →
RELEASE OF SECURITY INTEREST Recorded Aug 5, 2022
From: TRUIST BANK
To: DANIMER SCIENTIFIC, L.L.C.; DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.
Reel/Frame 061088/0018 →
SECURITY INTEREST Recorded May 3, 2021
From: DANIMER SCIENTIFIC, L.L.C.; DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.
To: TRUIST BANK
Reel/Frame 056122/0473 →
RELEASE OF SECURITY INTEREST Recorded Feb 5, 2021
From: WHITE OAK GLOBAL ADVISORS, LLC
To: DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.; DANIMER SCIENTIFIC, L.L.C.
Reel/Frame 055225/0196 →
SECURITY INTEREST Recorded Mar 19, 2019
From: DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.; DANIMER SCIENTIFIC, L.L.C.
To: SOUTHEAST COMMUNITY DEVELOPMENT FUND X, L.L.C.
Reel/Frame 048639/0606 →
SECURITY INTEREST Recorded Mar 15, 2019
From: DANIMER BIOPLASTICS, INC.; MEREDIAN, INC.; MEREDIAN BIOPLASTICS, INC.; DANIMER SCIENTIFIC, L.L.C.
To: WHITE OAK GLOBAL ADVISORS, LLC
Reel/Frame 048612/0195 →
RELEASE OF SECURITY INTEREST Recorded Feb 4, 2019
From: UNITED NATIONAL BANK
To: MEREDIAN, INC.
Reel/Frame 048234/0522 →