Methods and apparatuses for analyzing polynucleotide sequences
View Patent ↗Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
1. A surface for nucleic acid sequencing, said surface comprising a plurality of chemical layers including a poly(dimethylsiloxane) layer, a poly(ethylene glycol) layer, a polyelectrolyte multilayer comprising a tunable negatively charged final layer, a nucleic acid linkage layer and a nucleic acid layer,
wherein said poly(ethylene glycol) layer is positioned between said poly(dimethylsiloxane) layer and said polyelectrolyte multilayer, and
wherein said nucleic acid linkage layer attaches said nucleic acid layer to said polyelectrolyte multilayer.
2. The surface of claim 1 wherein said nucleic acid layer comprises a plurality of polynucleotides and wherein each polynucleotide is individually optically resolvable.
3. The surface of claim 2 wherein said nucleic acid linkage layer comprises biotin-streptavidin or digoxygenin-antidigoxygenin.
4. The surface of claim 1 wherein said poly(ethylene glycol) is diacrylated poly(ethylene glycol).