Inhibition of pathogens by probiotic bacteria
Compositions containing a lactic acid-producing bacterial strain, e.g., Bacillus coagulans for inhibition of pathogenic bacterial infections. Spores or extracellular products produced by the bacterial strains are also useful as inhibitory agents.
1 . A composition comprising an isolated Bacillus strain, wherein said strain (a) produces lactic acid, (b) has an optimal growth temperature of in the range of 20-44° C., and (c) grows in a pH range of 2-5.
2 . The composition of claim 1 , wherein said strain produces L(+) dextrorotatory lactic acid and produces spores resistant to temperatures of up to approximately 90° C.
3 . The composition of claim 1 , wherein said strain is selected from the group consisting of Bacillus coagulaus, Bacillus stereothermophilus, Bacillus thermoacidurans, Lactobacillus sporogenes, Bacillus smithii, Bacillus dextrolacticus, Lactobacillus cereale , and Bacillus recemilacticus.
4 . The composition of claim 1 , wherein said strain is Bacillus coagulans.
5 . The composition of claim 1 , wherein said strain is selected from the group consisting of Bacillus coagulans GBI-1, Bacillus coagulans GBI-20, Bacillus coagulans GBI-30 and Bacillus coagulans GBI-40.
6 . (canceled)
7 . A composition comprising an isolated Bacillus strain, wherein said strain produces lactic acid and has an optimal growth temperature of in the range of 20-25° C.
8 . The composition of claim 7 , wherein said strain produces L(+) dextrorotatory lactic acid and produces spores resistant to temperatures of up to approximately 90° C.
9 . The composition of claim 7 , wherein said strain is selected from the group consisting of Bacillus coagulans, Bacillus stereothermophilus, Bacillus thermoacidurans, Lactobacillus sporogenes, Bacillus smithii, Bacillus dextrolacticus, Lactobacillus cereale , and Bacillus recemilacticus.
10 . The composition of claim 7 , wherein said strain is Bacillus coagulans.
11 . The composition of claim 7 , wherein said strain is Bacillus coagulans GBI-20.
12 . (canceled)
13 . A composition comprising an isolated Bacillus strain, wherein said strain produces lactic acid and has an optimal growth temperature of in the range of 25-35° C.
14 . The composition of claim 13 , wherein said strain produces L(+) dextrorotatory lactic acid and produces spores resistant to temperatures of up to approximately 90° C.
15 . The composition of claim 13 , wherein said strain is selected from the group consisting of Bacillus coagulans, Bacillus stereothermophilus, Bacillus thermoacidurans, Lactobacillus sporogenes, Bacillus smithii, Bacillus dextrolacticus, Lactobacillus cereale , and Bacillus recemilacticus.
16 . The composition of claim 13 , wherein said strain is Bacillus coagulans.
17 - 18 . (canceled)
19 . A composition comprising an isolated Bacillus strain, wherein said strain produces lactic acid and has an optimal growth temperature of in the range of 35-40° C.
20 . The composition of claim 19 , wherein said strain produces L(+) dextrorotatory lactic acid and produces spores resistant to temperatures of up to approximately 90° C.
21 . The composition of claim 19 , wherein said strain is selected from the group consisting of Bacillus coagulans, Bacillus stereothermophilus, Bacillus thermoacidurans, Lactobacillus sporogenes, Bacillus smithii, Bacillus dextrolacticus, Lactobacillus cereale , and Bacillus recemilacticus.
22 . The composition of claim 19 , wherein said strain is Bacillus coagulans.
23 . (canceled)
24 . An extracellular product derived from the composition of claim 19 .
25 . A method of inhibiting a pathogenic bacterial infection, comprising contacting an infected site with the composition of claim 1 .
26 . A method of inhibiting a pathogenic bacterial infection, comprising contacting an infected site with a Bacillus coagulans composition.
27 - 35 . (canceled)