In vivo production of cyclic peptides
View Patent ↗The present invention relates ti methods and compositions utilizing inteins to generated libraries of cyclic peptides in vivo.
1. An isolated cell comprising:
a cyclic peptide consisting of three amino acids, wherein at least one of said amino acids is a Ser, Thr or Cys.
2. The isolated cell of claim 1 , wherein said cell further comprises:
a nucleic acid encoding a fusion protein comprising, in order:
a) a C-terminal intein domain;
b) a peptide; and
c) a N-terminal intein domain;
wherein said fusion protein undergoes a reaction to produce said cyclic peptide.
3. The isolated cell of claim 1 , wherein said cell is a mammalian cell.
4. The isolated cell of claim 2 , wherein said fusion protein is encoded by retroviral vector.
5. The isolated cell of claim 2 , wherein said cell is a mammalian cell.
6. An isolated cell comprising a cyclic peptide consisting of three amino acids, wherein at least one of said amino acids is a Ser, Thr or Cys, and wherein said cyclic peptide is produced as a result of a cyclization reaction of a fusion protein comprising, in order: a) a C-terminal intein domain; b) a peptide; and c) a N-terminal intein domain.
7. The isolated cell of claim 6 , wherein said cell is a mammalian cell.
8. The isolated cell of claim 6 , wherein said fusion protein is encoded by retroviral vector.