Reactive ion etched substrates and methods of making and using
View Patent ↗Disclosed herein are substrates comprising reactive ion etched surfaces and specific binding agents immobilized thereon. The substrates may be used in methods and devices for assaying or isolating analytes in a sample. Also disclosed are methods of making the reactive ion etched surfaces.
1. A substrate comprising
at least one reactive ion etched polymethylmethacrylate or cyclo-olefin surface having at least one polypeptide bound directly thereon;
at least one unetched polymethylmethacrylate or cyclo-olefin surface which fails to bind the polypeptide directly thereon when contacted therewith; and
at least one reactive ion etched or unetched surface of a second polymer wherein the reactive ion etched surface of the second polymer fails to bind the polypeptide directly thereon when contacted therewith;
wherein the polypeptide specifically binds a ligand of interest in a sample to be assayed; and wherein said binding directly thereon is in the absence of a linker.
2. The substrate of claim 1 , wherein at least one of the polymethylmethyacrylate or cyclo-olefin surfaces is adjacent to at least one of the surfaces of the second polymer.
3. The substrate of claim 1 , wherein the polypeptide is an antigen or an antibody.
4. The substrate of claim 1 , wherein the second polymer is polycarbonate.
5. The substrate of claim 1 , wherein the unetched surface of the second polymer has a second polypeptide bound directly thereon which second polypeptide is the same as or different from the polypeptide bound directly thereon the reactive ion etched polymethylmethacrylate or cyclo-olefin surface.
6. The substrate of claim 1 , wherein the reactive ion etched polymethylmethacrylate or cyclo-olefin surface is in a pattern on the substrate.
7. The substrate of claim 1 , wherein the unetched surface is in a pattern on the substrate.
8. The substrate of claim 1 , wherein the first and second polymers are in a pattern.
9. The substrate of claim 1 , wherein the polypeptide is bound to the reactive ion etched polymethylmethacrylate or cyclo-olefin surface in the absence of a non-specific blocking buffer.
10. An assay device comprising the substrate of claim 1 operably attached to a housing.
11. A method of assaying or isolating a ligand in a sample which comprises contacting the substrate of claim 1 with the sample.
12. The method of claim 11 , which further comprises detecting or measuring the polypeptide complexed with the ligand or the ligand complexed with the polypeptide.
13. A kit comprising the substrate of claim 1 packaged together with reagents for assaying or isolating the ligand.