Dry powder cells and cell culture reagents and methods of production thereof
The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.
1 . A dry format cell culture medium supplement comprising at least one amino acid and a tribasic phosphate salt.
2 . The supplement according to claim 1 wherein the supplement is capable of dissolving to provide a concentration of at least one component of the supplement greater than the concentration of that component in the medium being supplemented.
3 . A method of culturing cells comprising growing cells in culture medium; preparing a supplement comprising at least one amino acid and a tribasic phosphate salt; and adding said supplement to said growing cells in culture medium.