Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
View Patent ↗The present invention relates to pluripotent stem cells, particularly to pluripotent embryonic-like stem cells. The invention further relates to methods of purifying pluripotent embryonic-like stem cells and to compositions, cultures and clones thereof. The present invention also relates to a method of transplanting the pluripotent stem cells of the present invention in a mammalian host, such as human, comprising introducing the stem cells, into the host. The invention further relates to methods of in vivo administration of a protein or gene of interest comprising transfecting a pluripotent stem cell with a construct comprising DNA which encodes a protein of interest and then introducing the stem cell into the host where the protein or gene of interest is expressed. The present also relates to methods of producing mesodermal, endodermal or ectodermal lineage-committed cells by culturing or transplantation of the pluripotent stem cells of the present invention.
1. A method of providing a host with isolated cells derived from postnatal human tissues, the method comprising administering into the host the isolated cells, wherein the isolated cells express SSEA4, do not express CD34, have a normal karyotype, do not form tumors in the host, are capable of self-renewal, and are capable of differentiation to cells of endodermal, ectodermal and mesodermal lineages, said isolated cells having been produced by expanding, in culture, a cultured cell clone from a postnatal human tissue.
2. The method of claim 1 wherein the isolated cells are administered in combination with one or more proliferation factors or lineage commitment factors.
3. The method of claim 1 wherein the expansion is greater than 50 cell doublings.
4. The method of claim 1 wherein the expansion comprises cell doublings of between 12 and 47.
5. The method of claim 1 wherein the isolated cells have been passaged and cryopreserved after passage prior to introduction to the host.