Cell culture media
The invention is a cell culture medium that can include reduced or no serum and that enhances the performance of serum-free media for cell culture. The medium supports the growth of cells for both small scale and large scale propagation of cells. The invention also includes a method of cultivating cells using the cell culture medium of the present invention.
1 . A cell culture media comprising the following components: basal media, approximately 1-4 mM glutamine, approximately 0.3-2% albumin, approximately 5-15 mg/L insulin, approximately 0.5-9 mg/L transferrin, approximately 5-15 uM ethanolamine, and approximately 1-3% of a purified lipoprotein material.
2 . A cell culture media comprising the following components: basal media, sodium selenite, albumin, insulin, transferrin, peptone, fetuin, vitamin E and a purified lipoprotein material.
3 . The cell culture media of claim 1 or 2 wherein the purified lipoprotein material is derived from bovine serum or plasma.
4 . The cell culture media of claim 1 or 2 wherein the purified lipoprotein material is cholesterol-rich.
5 . The cell culture media of claim 1 or 2 wherein the purified lipoprotein material is produced by contacting serum or plasma with a silica adsorbant.
6 . A method to culture cells comprising incubating the cells in a culture media consisting essentially of the following components: basal media, glutamine, albumin, insulin, transferrin, ethanolamine, and a purified lipoprotein material.
7 . A method to culture cells comprising incubating the cells in a culture media comprising the following components: basal media, sodium selenite, albumin, insulin, transferrin, peptone, fetuin, vitamin E and a purified lipoprotein material.
8 . The method of claim 6 or 7 wherein the purified lipoprotein material is derived from bovine serum or plasma.
9 . The method of claim 6 or 7 wherein the purified lipoprotein material is cholesterol-rich.
10 . The method of claim 6 or 7 wherein the purified lipoprotein material is produced by contacting serum or plasma with a silica adsorbant.
11 . The method of claim 6 wherein the cells produce a peptide.
12 . The method of claim 11 wherein the peptide is an antibody.
13 . The method of claim 11 wherein the peptide is an immunoglobulin.
14 . The method of claim 6 wherein the cells are hybridoma cells.
15 . The method of claim 7 wherein the cells are cancer cells.
16 . The method of claim 7 wherein the cells are in suspension.
17 . The method of claim 7 wherein the cells are adherent.
18 . The method of claim 7 , wherein the cells are selected from the group consisting of K562 cells, HL-60 cells, Daudi cells, HeLA cells, THP-1 cells, Jurkat cells and EL4 cells.
19 . The method of claim 7 wherein the cells are human cells.
20 . A method for culturing hybridoma cells in vitro comprising incubating the cells in a cell culture media comprising the following components: basal media, glutamine, albumin, insulin, transferrin, ethanolamine, and a purified lipoprotein material.
21 . A cell culture media consisting essentially of the following components: basal media, glutamine, albumin, insulin, transferrin, ethanolamine, and a purified lipoprotein material.