Enhanced secretion/retention of growth hormone releasing hormone (GHRH) from muscle cells by species-specific signal peptide
View Patent ↗One aspect of the current invention is a method designing and using species-specific or synthetic signal peptides and GHRH sequences for the purpose of preventing and/or treating chronic illness in a subject by utilizing methodology that administers a single dose of nucleic acid expression vector or nucleic acid expression construct encoding a GHRH or functional biological equivalent to a subject through a parenteral route of administration.
1. A method of expressing and secreting an encoded growth-hormone-releasing-hormone (“GHRH”) peptide from a cell of a subject comprising; delivering into the cell of the subject an isolated nucleic acid expression construct that comprises a promoter operatively linked to a nucleic acid sequence encoding a signal peptide coupled to the encoded GHRH peptide, wherein the encoded signal peptide has the amino acid sequence of SEQ ID NO:51 and the encoded GHRH has the amino acid sequence of SEQ ID NO:10, wherein the delivering into the cell of the subject the nucleic acid expression construct initiates expression of the encoded signal peptide and GHRH.
2. The method of claim 1 , wherein delivering comprises: an electroporation method in conjunction with a carrier.
3. The method of claim 2 , wherein the electroporation method comprises:
a. penetrating a tissue in the subject with a plurality of needle electrodes, wherein the plurality of needle electrodes are arranged in a spaced relationship;
b. introducing the nucleic acid expression construct into the tissue between the plurality of needle electrodes; and
c. applying an electrical pulse to the plurality of needle electrodes.
4. The method of claim 3 , wherein the nucleic acid expression construct is delivered in a single dose.
5. The method of claim 4 , wherein the single dose comprises about a 0.01-10 mg quantity of nucleic acid expression construct.
6. The method of claim 2 , wherein the carrier further comprises, a transfection-facilitating polypeptide.
7. The method of claim 6 , wherein the transfection-facilitating polypeptide comprises a charged polypeptide.
8. The method of claim 7 , wherein the transfection-facilitating polypeptide comprises poly-L-glutamate.
9. The method of claim 1 , wherein the cell of the subject comprise diploid cells.
10. The method of claim 1 , wherein the cell of the subject comprise muscle cells.
11. The method of claim 1 , wherein the subject comprises human, ruminant animal, food animal, or work animal.
12. An isolated nucleic acid molecule comprising a sequence that encodes a signal peptide coupled to an encoded growth-hormone-releasing-hormone (“GHRH”) peptide, wherein the encoded signal peptide has the amino acid sequence of SEQ ID NO:51 and the encoded GHRH has the amino acid sequence of SEQ ID NO:10.
13. A method of secreting an encoded growth-hormone-releasing-hormone (“GHRH”) peptide from a cell of a subject comprising:
a. penetrating a tissue in the subject with a plurality of needle electrodes, wherein the plurality of needle electrodes are arranged in a spaced relationship;
b. introducing a single dose of an isolated nucleic acid expression construct into the tissue between the plurality of needle electrodes, wherein the single dose comprises a transfection-facilitating polypeptide; and
c. applying an electrical pulse to the plurality of needle electrodes; wherein, the isolated nucleic acid construct comprises a promoter sequence operatively linked to a sequence that encodes a signal peptide coupled to an encoded growth-hormone-releasing-hormone (“GHRH”) peptide, wherein the encoded signal peptide has the amino acid sequence of SEQ ID NO:51 and the encoded GHRH has the amino acid sequence of SEQ ID NO:10; the transfection-facilitating polypeptide comprises poly-L-glutamate; and the subject comprises human, ruminant animal, food animal, or work animal.