Detection and quantification of analytes in solution using polymers
View Patent ↗The invention relates to methods and systems for identifying, quantitating and/or analyzing analytes from samples. The analytes may be organic or inorganic in nature and include but are not limited to pathogens such as viruses.
1. A method for detecting an analyte in a sample comprising
contacting a sample with a polymer having bound to it a plurality of analyte-specific binding partners,
detecting binding of an analyte to at least one of the plurality of analyte-specific binding partners, and
determining a labeling pattern of the polymer while the plurality of analyte-specific binding partners is bound to the polymer,
wherein the labeling pattern of the polymer is a spatial pattern of detectable labels along the length of the polymer that indicates the identity of the analyte, and wherein the polymer is a non-naturally occurring nucleic acid.
2. The method of claim 1 , wherein the analyte is a plurality of analytes, the polymer is a plurality of polymers, and the analyte-specific partner is a plurality of analyte-specific polymers.
3. The method of claim 1 , wherein the analyte-specific binding partner is an antibody.
4. The method of claim 1 , wherein binding of the analyte to the analyte specific binding partner is detected using a secondary analyte-specific binding partner.
5. The method of claim 4 , wherein the secondary analyte-specific binding partner is conjugated to a detectable label.
6. The method of claim 4 , wherein the analyte-specific binding partner and the secondary analyte-specific binding partner is each labeled with a member of a FRET pair.
7. The method of claim 1 , wherein the analyte is directly detectable and binding of the analyte to the analyte-specific binding partner is directly detected.
8. The method of claim 1 , wherein the labeling pattern of the polymer is a spatial pattern of one or more sequence-specific probes bound along the length of the polymer.
9. The method of claim 1 , wherein the detectable labels are conjugated to one or more sequence-specific probes.
10. The method of claim 1 , wherein the detectable labels are incorporated into the polymer.
11. The method of claim 1 , further comprising quantifying analyte concentration in the sample by determining an amount of analyte bound to the plurality of analyte-specific binding partners and comparing with a standard curve.
12. The method of claim 1 , further comprising harvesting an analyte-bound polymer.
13. The method of claim 12 , further comprising analyzing the analyte bound to the polymer.
14. The method of claim 1 , wherein the analyte is a nucleic acid, a carbohydrate, a protein, a peptide, a lipid, a toxin, a cell, a spore, a cellular fragment or a spore fragment.
15. The method of claim 1 , wherein the polymer is elongated prior to or simultaneously with determining the labeling pattern of the polymer.
16. The method of claim 1 , wherein the labeling pattern of the polymer is a spatial pattern of analyte-specific binding partners bound along the length of the polymer.
17. The method of claim 1 , wherein the labeling pattern of the polymer is determined using a focused flow through an electric field.
18. A method for detecting an analyte in a sample comprising
contacting a sample with a polymer having bound to it an analyte-specific binding partner,
detecting binding of an analyte to the analyte-specific binding partner, and
determining a labeling pattern of the polymer while the plurality of analyte-specific binding partners is bound to the polymer,
wherein the labeling pattern of the polymer indicates the identity of the analyte, and the polymer is a non-naturally occurring nucleic acid that is at least 80 kilobases in length.
19. A method for detecting an analyte in a sample comprising
contacting a sample with a polymer having bound to it a plurality of analyte-specific binding partners,
detecting binding of an analyte to at least one of the plurality of analyte-specific binding partners, and
determining a labeling pattern of the polymer while the plurality of analyte-specific binding partners is bound to the polymer,
wherein the polymer is a non-naturally occurring nucleic acid, the labeling pattern of the polymer is a spatial pattern of analyte-specific binding partners bound along the length of the polymer or one or more sequence-specific probes bound along the length of the polymer, and the labeling pattern of the polymer indicates the identity of the analyte.