Micro-volume blood sampling device
The invention features a single tool for collecting a known volume of a fluid biological sample from an animal, such as a human or small mammal, for mixing the sample with a predetermined amount of a reagent or a diluent, physically separate components of the sample, for storing the sample in a secure and stable fashion, and for then recovering some or all of the sample in a condition suitable for performing a diagnostic assay. The tool can be equipped with an optional means of recording or displaying information about the sample, such as a tag, a bar code, or a surface indicator that identifies, e.g., the date, animal number, and any additional information necessary. A second bar coded label to remove and attaché to the cage shall be provided. The cage/blood-sample can be correlated if needed at a later date.
1 . A sample collection device comprising:
a) a receptacle body portion having a sample port;
b) a sample chamber having an interior surface;
c) a fluid conduit, positioned at least partially in said receptacle body, fluidly connecting the sample port with the sample chamber; and
d) a fluid reservoir in fluid communication with said sample chamber; and
e) a vent port in atmospheric communication with said sample port.
2 . The device of claim 1 , wherein said device further comprises a vent plug in operable alignment with said vent port.
3 . The device of claim 1 , wherein said device further comprises means for attaching said receptacle body to a centrifuge.
4 . The device of claim 1 , wherein said receptacle body portion further comprises a cavity in fluid communication with said sample chamber and centrifugally distal to said conduit.
5 . The device of claim 1 , further comprising a solute in said fluid reservoir.
6 . The device of claim 5 , wherein said solute is a dilution fluid.
7 . The device of claim 5 , wherein said solute further comprises a stabilizing reagent.
8 . The device of claim 5 , wherein said solute further comprises an analyte detection reagent.
9 . The device of claim 1 , wherein said device comprises a plurality of fluid reservoirs.
10 . The device of claim 1 , wherein said device further comprises a dry reagent on at least a portion of said interior surface of said sample chamber.
11 . The device of claim 1 , wherein said fluid conduit has a volume, and sample collection device has a sample collection capacity approximately equivalent to said fluid conduit volume.
12 . The device of claim 1 , wherein said sample chamber has an interior surface, at least a portion of said interior surface being hydrophobic.
13 . The device of claim 1 , wherein said fluid conduit comprises an interior surface characterized as being hydrophilic.
14 . A sample collection device comprising a conduit portion and a receptacle body portion in fluid communication with said conduit portion, said conduit portion having a volume of less than 200 microliters, and said receptacle body portion comprising, affixed to said receptacle body portion:
a) means for allowing a fluid sample to enter said receptacle portion via said conduit portion; and
b) means for forcing said fluid sample to exit said receptacle portion via said conduit portion.
15 . The sample collection device of claim 14 , further comprising a means for attaching said device to a centrifuge.
16 . The sample collection device of claim 14 , wherein said receptacle body portion further comprises a fluid reservoir, a resident fluid within said fluid reservoir, and means for allowing said resident fluid to mix with said fluid sample.
17 . The sample collection device of claim 14 , wherein said receptacle body portion further comprises a means for sequestering a separable component from said fluid sample.
18 . The sample collection device of claim 14 , wherein said receptacle body portion comprises an interior space having an interior pressure, and further comprises a means for maintaining said interior pressure at atmospheric pressure.
19 . The sample collection device of claim 18 , wherein said receptacle body further comprises means for increasing and decreasing said interior pressure.
20 . The sample collection device of claim 18 , wherein said receptacle body portion further comprises means for maintaining said interior pressure at atmospheric pressure.
21 . The sample collection device of claim 18 , wherein said fluid sample has a vapor phase, and wherein said receptacle body further comprises means for preventing said vapor phase from exiting said device.
22 . A method for collecting and recovering a liquid sample, said method comprising the steps of:
a) contacting a liquid sample with the sample collection device of claim 14 so as to allow a known volume of said sample to enter said receptacle body portion of said device;
b) subjecting said sample collection device to centrifugal force; and
c) applying pressure to said receptacle body portion of said device, so as to extrude said sample from said device.