C. elegans p21-activated kinase (PAK) gene and associated loss-of-function phenotypes that facilitate screening for small molecule modulators of PAK activity in the nematode, caenorhabditis elegans
View Patent ↗The invention refers to a novel C. elegans p21-activated kinase gene, the pak-3 gene, and associated loss-of-function phenotypes. These phenotypes can be used to elucidate PAK signaling pathways in C. elegans and to screen compounds that modulate PAK signaling.
1. An isolated polynucleotide comprising a DNA sequence selected from one of the following groups:
a] a DNA sequence of SEQ ID NO. 1; or
b] a DNA sequence which is complementary to SEQ ID NO. 1; or
c] a DNA sequence which is degenerate as a result of the genetic code to the DNA sequence of SEQ ID NO. 1.
2. An isolated polynucleotide comprising a polynucleotide sequence of SEQ ID NO. 1.
3. A recombinant vector sequence comprising SEQ ID NO. 1.
4. An isolated host cell comprising the recombinant vector of claim 3 .
5. The isolated host cell of claim 4 which is a cell of E. coli or of Saccharomyces cerevisiae.
6. A method of manufacturing an RNA molecule wherein the recombinant vector of claim 3 is transformed into a bacterial strain, the RNA is transcribed from the vector and the transcribed RNA is isolated and/or purified.
7. An RNA molecule obtained by the method of claim 6 .