Polypeptide from a cellulolytic fungus having cellulolytic enhancing activity
View Patent ↗The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. An isolated polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence which has at least 95% sequence identity to amino acids 20 to 326 of SEQ ID NO: 2;
(b) a polypeptide which is encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) nucleotides 388 to 1332 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 388 to 1332 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and
(c) a polypeptide which is encoded by a polynucleotide comprising a nucleotide sequence which has at least 95% sequence identity to nucleotides 388 to 1332 of SEQ ID NO: 1.
2. The polypeptide of claim 1 , which comprises the amino acid sequence of SEQ ID NO: 2.
3. The polypeptide of claim 1 , which comprises amino acids 20 to 326 of SEQ ID NO: 2.
4. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pEJG120 which is contained in E. coil NRRL B-30699.
5. A detergent composition comprising the polypeptide having cellulolytic enhancing activity of claim 1 , a polypeptide having cellulolytic activity, and a surfactant.
6. The polypeptide of claim 1 , comprising an amino acid sequence which has at least 95% sequence identity to amino acids 20 to 326 of SEQ ID NO: 2.
7. The polypeptide of claim 6 , comprising an amino acid sequence which has at least 97% sequence identity to amino acids 20 to 326 of SEQ ID NO: 2.
8. The polypeptide of claim 1 , which is encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) nucleotides 388 to 1332 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 388 to 1332 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
9. The polypeptide of claim 1 , which is encoded by a polynucleotide which has at least 95% sequence identity to nucleotides 388 to 1332 of SEQ ID NO: 1.
10. The polypeptide of claim 9 , which is encoded by a polynucleotide which has at least 97% sequence identity to nucleotides 388 to 1332 of SEQ ID NO: 1.
11. The polypeptide of claim 2 , which consists of the amino acid sequence of SEQ ID NO: 2.
12. The polypeptide of claim 3 , which consists of amino acids 20 to 326 of SEQ ID NO: 2.
13. The polypeptide of claim 1 , which is encoded by a polynucleotide which comprises SEQ ID NO: 1.
14. The polypeptide of claim 1 , which is encoded by a polynucleotide which comprises nucleotides 388 to 1332 of SEQ ID NO: 1.
15. The polypeptide of claim 13 , which is encoded by a polynucleotide which consists of SEQ ID NO: 1.
16. The polypeptide of claim 14 , which is encoded by a polynucleotide which consists of nucteotides 388 to 1332 of SEQ ID NO: 1.