Geranyl diphosphate synthase molecules, and nucleic acid molecules encoding same
View Patent ↗In one aspect, the present invention provides isolated nucleic acid molecules that each encode a geranyl diphosphate synthase protein, wherein each isolated nucleic acid molecule hybridizes to a nucleic acid molecule consisting of the sequence set forth in SEQ ID NO:1 under conditions of 5×SSC at 45° C. for one hour. The present invention also provides isolated geranyl diphosphate synthase proteins, and methods for altering the level of expression of geranyl diphosphate synthase protein in a host cell.
1. An isolated nucleic acid molecule comprising a sequence of nucleotides selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO:2;
(b) a nucleotide sequence having at least 99% identity to SEQ ID NO:2;
(c) the nucleotide sequence set forth in SEQ ID NO:9; and
(d) a nucleotide sequence having at least 99% identity to SEQ ID NO:9;
wherein said molecule encodes a protein having geranyl diphosphate synthase activity.
2. The isolated nucleic acid molecule of claim 1 comprising a sequence of nucleotides selected from the group consisting of SEQ ID NO:2 and a nucleotide sequence having at least 99% identity to SEQ ID NO:2.
3. The isolated nncleic acid molecule of claim 1 comprising a sequence of nucleotides selected from the group consisting of SEQ ID NO:9 and a nucleotide sequence having at least 99% identity to SEQ ID NO:9.
4. The isolated rmcleic acid molecule of claim 1 comprising the nucleic acid sequence set forth in SEQ ID NO:2.
5. The isolated nucleic acid molecule of claim 1 consisting of the nucleic acid sequence set forth in SEQ ID NO:2.
6. A vector comprising the nucleic acid molecule of claim 1 .
7. The vector of claim 6 wherein said vector is an expression vector.
8. An isolated host cell comprising the vector of claim 6 .
9. The host cell of claim 8 wherein the host cell is a prokaryotic cell.
10. The host cell of claim 8 wherein the host cell is a eukaryotic cell.
11. The host cell of claim 8 wherein the host cell is a plant cell.
12. The plant cell of claim 11 wherein the plant cell is a cell of a plant of the genus Abies.
13. The isolated nucleic acid molecule of claim 1 comprising the nucleic acid sequence set forth in SEQ ID NO:9.
14. The isolated nucleic acid molecule of claim 3 comprising a sequence of nucleotides having at least 99% identity to SEQ ID NO:9.