Synovial fluid barrier
View Patent ↗A composite tissue formed in situ is provided. The composite tissue includes a synovial joint tissue; and a barrier material adhered thereto for sealing the synovial joint tissue against synovial fluid. Also provided is a method for regenerating synovial joint tissue in situ by excluding synovial fluid therefrom. The method includes providing a synovial joint tissue having a defect; and placing a barrier material in intimate contact with the defect for sealing the defect against synovial fluid. The barrier material includes a curable protein copolymer. The method further includes curing the protein copolymer in situ. The barrier material can include a crosslinked network, or a self gelled network of repeating elastin-like and fibroin-like polymer chains.
1. An in situ method of treating a wound in synovial joint tissue comprising the steps of:
(a) applying to the tissue in the presence of synovial fluid:
(i) a crosslinkable protein copolymer comprising recombinant elastin and fibroin copolymer chains; and
(ii) a crosslinker;
(b) allowing the recombinant elastin and fibroin copolymer chains to crosslink in situ, thereby forming an adherent barrier coating on the synovial tissue surface, wherein the coating excludes at least a portion of the synovial fluid from the tissue, and forms an effective barrier on the synovial joint tissue surface against synovial fluid wherein said coating exclusion results in formation of the barrier coating, and
(c) seeding the barrier coating with autologous cells which promote the wound healing and permit said cells to infiltrate the wound beneath the barrier coating to improve regenerative healing.
2. The method of claim 1 , wherein the crosslinker is an isocyanate.
3. The method of claim 2 , wherein the isocyanate is hexamethylene diisocyanate.
4. The method of claim 1 , wherein applying includes injection into a site of the wound.
5. The method of claim 1 , further including accessing pluripotential mesenchymal stem cells in bone proximal to the wound.
6. The method of claim 1 , wherein the synovial joint tissue is cartilage.
7. The method of claim 1 , wherein the synovial joint tissue is a ligament.
8. The method of claim 1 , wherein a synovial joint tissue is the facet joint of the spine.