Prefilter system for biological systems
View Patent ↗The present invention is to a prefilter for purification systems such as affinity chromatography columns and ultrafiltration systems. The prefilter, positioned upstream of the system inlet, reduces the presence of non-specific binding (NSB) species that enter the system, thereby extending the yield, capacity and lifetime of the system. Suitable agents include but are not limited to hydrophobic entities; lipophilic entities; activated carbon; charged cation or anion entities; ligands; particles such as fumed silica, glass, controlled pore glass or derivitized version of each; silica or silicates; and combinations thereof. The material(s) can be incorporated into a variety of media such as fibers, beads, membranes and the like and then incorporated into a variety of device designs including lenticular pads, depth filters, bead containing columns, spiral wound devices, TFF devices and the like.
1. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass and the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device.
2. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass, the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device and wherein the media is a cellulose derived fibrous pad containing a material selected from the group consisting of glass, silicates, silica and blends thereof.
3. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass, the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device and wherein the media is a cellulose derived fibrous pad containing a material selected from the group consisting of glass, wherein the glass is in a form selected from the group consisting of fibers, mats and controlled pore glass particles.