Soluble interleukin-1 receptor accessory molecule
View Patent ↗The present invention relates to a novel soluble IL-1 receptor accessory molecule (IL-1R AcM) protein which is a member of the Ig superfamily. In particular, isolated nucleic acid molecules are provided encoding the human IL-1R AcM protein. IL-1R AcM polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. Screening methods are futher provided for identifying agonist and antagonists of IL-1 signal transduction. The invention further relates to methods for treating physiologic and pathologic disease conditions with IL-1R AcM antagonists.
1. An isolated polypeptide selected from the group consisting of:
(a) a polypeptide comprising amino acids −17 to 339 of SEQ ID NO:2;
(b) a polypeptide comprising amino acids 1 to 339 of sequence of SEQ ID NO:2;
(c) a polypeptide comprising the full-length IL-1R AcM polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97666; and
(d) a polypeptide comprising the mature IL-1R AcM polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97666.
2. The isolated polypeptide of claim 1 , wherein said polypeptide is (a).
3. The isolated polypeptide of claim 1 , wherein said polypeptide is (b).
4. The isolated polypeptide of claim 1 , wherein said polypeptide is (c).
5. The isolated polypeptide of claim 1 , wherein said polypeptide is (d).
6. The isolated polypeptide of claim 1 , wherein said polypeptide is glycosylated.
7. The isolated polypeptide of claim 1 , wherein said polypeptide is fused to a heterologous polypeptide.
8. An isolated polypeptide produced by a method comprising:
(a) expressing the polypeptide of claim 1 by a cell; and
(b) recovering said polypeptide.
9. An isolated polypeptide selected from the group consisting of:
(a) a polypeptide consisting of amino acids −17 to 339 of SEQ ID NO:2;
(b) a polypeptide consisting of amino acids 1 to 339 of sequence of SEQ ID NO:2;
(c) a polypeptide consisting of the full-length IL-1R AcM polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97666; and
(d) a polypeptide consisting of the mature IL-1R AcM polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97666.
10. The isolated polypeptide of claim 9 , wherein said polypeptide is (a).
11. The isolated polypeptide of claim 9 , wherein said polypeptide is (b).
12. The isolated polypeptide of claim 9 , wherein said polypeptide is (c).
13. The isolated polypeptide of claim 9 , wherein said polypeptide is (d).
14. The isolated polypeptide of claim 9 , wherein said polypeptide is glycosylated.
15. The isolated polypeptide of claim 9 , wherein said polypeptide is fused to a heterologous polypeptide.
16. An isolated polypeptide produced by a method comprising:
(a) expressing the polypeptide of claim 9 by a cell; and
(b) recovering said polypeptide.