Treatments for contaminant reduction in lactoferrin preparations and lactoferrin containing compositions
View Patent ↗A method of preparing an ultra-cleansed lactoferrin preparation, termed treatment for contaminant reduction (TCR) is provided which includes the steps of treating commercial lactoferrin preparation with at least one each of surfactants, antioxidants and polyphenols to form purified lactoferrin (LF-TCR) and drying the LF-TCR. Additionally a therapeutic lactoferrin composition is provided which contains LF-TCR and optionally surfactants, antioxidants, polyphenols, tissue/membrane diffusion facilitating agents and anionic compounds. The therapeutic lactoferrin composition can additionally contain bioactive agents, dietary supplements, nutraceuticals/functional foods, prophylactic agents, therapeutic agents and probiotic lactic acid bacteria.
1. A method of preparing an ultra-cleansed lactoferrin preparation, termed treatment for contaminant reduction (TCR) comprising:
treating said commercial lactoferrin preparation with at least one surfactant;
contacting said surfactant-treated lactoferrin with at least one antioxidant;
purifying said antioxidant-treated lactoferrin with at least one polyphenol to form purified lactoferrin (LF-TCR); and
drying said LE-TCR.
2. The method according to claim 1 wherein said ultra-cleansed lactoferrin preparation is purified from commercially available lactoferrin isolated from dairy sources selected from the group consisting of colostrum, milk, whey and milk serum.
3. The method according to claim 2 wherein said dairy sources are obtained from mammals selected from the group consisting of humans, cows, buffalos, horses, sheep, pigs and camels.
4. The method according to claim 1 wherein said ultra-cleansed lactoferrin preparation is purified from recombinant sources and genetically-modified organisms (GMOs).
5. The method according to claim 1 wherein said at least one surfactant is selected from the group consisting of food-grade detergents, bile salts and plant saponins.
6. The method according to claim 1 wherein the concentration of said surfactant in said treating step is between approximately 0.01 mg and 1,000.00 mg for each 100.00 mg of lactoferrin.
7. The method according to claim 1 wherein said at least one antioxidant is selected from the group consisting of Vitamin A, Vitamin C, Vitamin E and metal chelators.
8. The method according to claim 1 wherein the concentration of said antioxidant in said contacting step is between approximately 0.01 mg and 10,000.00 mg for each 100.00 mg of lactoferrin.
9. The method according to claim 1 wherein said at least one polyphenol is selected from the group consisting of oleoresins, aquaresins, terpenes, flavonoids and biliproteins.
10. The method according to claim 1 wherein the concentration of said polyphenol in said purifying step is between approximately 0.01 mg and 10,000.00 mg for each 100.00 mg of lactoferrin.
11. The method according to claim 1 further comprising including an anionic compound during said contacting step.
12. The method according to claim 11 wherein said anionic compound is selected from the group consisting of a carbonate, a bicarbonate or a carbonated liquid.