Anti-neovasculature preparations for cancer
Disclosed herein are immunogenic compositions, methods of designing immunogenic compositions, methods of treatment using immunogenic compositions, methods of evaluating cell-mediated immunity resulting from immunogenic compositions, research models, and methods of making research models, all of which relate to targeting tumor vasculature.
1 . A method of treating neoplastic disease comprising the step of immunizing a mammal to induce a cellular immune response directed against a first antigen differentially expressed by tumor-associated neovasculature and a second antigen associated with a tumor, wherein the immunization comprises delivering at least a first immunogen corresponding to the first antigen and a second immunogen corresponding to the second antigen.
2 . The method of claim 1 , wherein the cellular immune response comprises a CTL response.
3 . The method of claim 1 further comprising the step of detecting the cellular immune response.
4 . The method of claim 3 , wherein the detecting step comprises detection of tumor growth inhibition, tumor size reduction, inhibition of tumor metastasis, or increase in life expectancy of the mammal.
5 . The method of claim 3 , wherein the detecting step comprises an assay selected from the group consisting of a cytokine assay, a chromium release assay, an immunofluorescence assay, a cytotoxic T lymphocyte (CTL) assay, an Elispot assay, and observation of the health of the mammal.
6 . A method of treating neoplastic disease comprising the step of immunizing a mammal to induce a cellular immune response directed against an antigen differentially expressed by tumor-associated neovasculature, wherein the immunization comprises delivering a first immunogen comprising at least one housekeeping epitope and a second immunogen comprising at least one immune epitope, wherein the housekeeping and immune epitopes are derived from said antigen differentially expressed by tumor-associated neovasculature.
7 . The method of claim 6 , wherein the first immunogen and the second immunogen are the same.
8 . The method of claim 6 , further comprising the step of treating the mammal with an anti-tumor therapy active directly against cancerous cells.
9 . The method of claim 8 , wherein the anti-tumor therapy comprises immunization against a tumor-associated antigen.
10 . The method of claim 6 , wherein the cellular immune response comprises a CTL response.
11 . The method of claim 6 , further comprising the step of detecting the cellular immune response.
12 . The method of claim 11 , wherein the detecting step comprises detection of tumor growth inhibition, tumor size reduction, inhibition of tumor metastasis, or increase in life expectancy of the mammal.
13 . The method of claim 11 , wherein the detecting step comprises an assay selected from the group consisting of a cytokine assay, a chromium release assay, an immunofluorescence assay, a cytotoxic T lymphocyte (CTL) assay, an Elispot assay, and observation of the health of the mammal.
14 . A method of treating neoplastic disease comprising the step of immunizing a mammal to induce a cellular immune response directed against an antigen differentially expressed by tumor-associated neovasculature, wherein the immunization comprises delivering an immunogen comprising at least one housekeeping epitope derived from said antigen differentially expressed by tumor-associated neovasculature.
15 . The method of claim 14 , wherein the cellular immune response comprises a CTL response.
16 . The method of claim 14 , further comprising the step of detecting the cellular immune response.
17 . The method of claim 16 , wherein the detecting step comprises detection of tumor growth inhibition, tumor size reduction, inhibition of tumor metastasis, or increase in life expectancy of the mammal.
18 . The method of claim 16 , wherein the detecting step comprises an assay selected from the group consisting of a cytokine assay, a chromium release assay, an immunofluorescence assay, a cytotoxic T lymphocyte (CTL) assay, an Elispot assay, and observation of the health of the mammal.