Modified pectins, compositions and methods related thereto
View Patent ↗The present invention provides compositions of modified pectin and for preparing and using them.
1. A process for manufacturing a biologically active filtered pectin, comprising: (a) passing an aqueous solution comprising 1-20 mg/mL modified or unmodified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of less than 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC 50 less than 200 μg/mL in a cancer cell apoptosis model; and (b) collecting said biologically active filtered pectin.
2. The process of claim 1 , wherein the pore size is about 0.2 μm.
3. The process of claim 1 , wherein the solution has a pectin concentration of 1 to 10 mg/mL.
4. The process of claim 1 , wherein the solution has a pH in the range of 2.5 to 7.5.
5. A process for manufacturing a partially de-esterified biologically active pectin, comprising:
(a) treating a first aqueous solution comprising 1-20 mg/ml modified or unmodified pectin with acid, base, or both to partially de-esterify the pectin,
(b) neutralizing the first aqueous solution to form a second aqueous solution comprising partially de-esterified pectin,
(c) passing the second aqueous solution comprising partially de-esterified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of less than 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises filtered partially de-esterified biologically active pectin having an average molecular weight from 10 kDa to 250 kDa and an IC 50 less than 200 μg/mL in a cancer cell apoptosis model, and wherein the degree of esterification in the resulting partially de-esterified biologically active pectin is between 10% and 60%, and
(d) collecting said filtered partially de-esterified biologically active pectin.
6. The process of claim 5 , wherein the pore size is about 0.2 μm.
7. The process of claim 5 , wherein the first aqueous solution comprises 1 to 10 mg/ml modified or unmodified pectin.
8. The process of claim 5 , wherein the first aqueous solution has a pH in the range of 2.5 to 7.5.
9. A process for manufacturing a biologically active filtered pectin, comprising: (a) passing an aqueous solution comprising 1-20 mg/mL modified or unmodified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of 0.1 μm to 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC 50 less than 200 μg/mL in a cancer cell apoptosis model; and (b) collecting said biologically active filtered pectin.
10. The process of claim 9 , wherein the pore size is 0.1 μm to 0.7 μm.
11. The process of claim 9 , wherein the pore size is about 0.2 μm.
12. The process of claim 9 , wherein the solution has a pectin concentration of 1 to 10 mg/mL.
13. The process of claim 9 , wherein the solution has a pH in the range of 2.5 to 7.5.
14. A process for manufacturing a partially de-esterified biologically active filtered pectin, comprising:
(a) treating a first aqueous solution comprising 1-20 mg/ml modified or unmodified pectin with acid, base, or both to partially de-esterify the pectin,
(b) neutralizing the first aqueous solution to form a second aqueous solution comprising partially de-esterified pectin,
(c) passing the second aqueous solution comprising partially de-esterified pectin and 7.5%-25% w/w ethanol through a tangential flow filter with a pore size of 0.1 μm to 1.0 μm to provide a permeate and a retentate, wherein said permeate comprises filtered partially de-esterified biologically active filtered pectin having an average molecular weight from 10 kDa to 250 kDa and an IC 50 less than 200 μg/mL in a cancer cell apoptosis model, and wherein the degree of esterification in the resulting partially de-esterified pectin is between 10% and 60%, and
(d) collecting said partially de-esterified biologically active filtered pectin.
15. The process of claim 14 , wherein the pore size is about 0.2 μm.
16. The process of claim 14 , wherein the first aqueous solution comprises 1 to 10 mg/ml modified or unmodified pectin.
17. The process of claim 14 , wherein the first aqueous solution has a pH in the range of 2.5 to 7.5.
18. The process of claim 1 , wherein the average molecular weight of the biologically active filtered pectin is selected from the group consisting of 50 kDa-200 kDa, 80 kDa-150 kDa, 70 kDa-150 kDa and 80 kDa-100 kDa.
19. The process of claim 5 , wherein the average molecular weight of the biologically active filtered pectin is selected from the group consisting of 50 kDa-200 kDa, 80 kDa-150 kDa, 70 kDa-150 kDa and 80 kDa-100 kDa.
20. The process of claim 9 , wherein the average molecular weight of the biologically active filtered pectin is selected from the group consisting of 50 kDa-200 kDa, 80 kDa-150 kDa, 70 kDa-150 kDa and 80 kDa-100 kDa.
21. The process of claim 14 , wherein the average molecular weight of the biologically active filtered pectin is selected from the group consisting of 50 kDa-200 kDa, 80 kDa-150 kDa, 70 kDa-150 kDa and 80 kDa-100 kDa.
22. The process of claim 1 , wherein the biologically active filtered pectin inhibits cancer cell proliferation in a cancer cell apoptosis model with an IC 50 less than 100 μg/mL.
23. The process of claim 5 , wherein the biologically active filtered pectin inhibits cancer cell proliferation in a cancer cell apoptosis model with an IC 50 less than 100 μg/mL.
24. The process of claim 9 , wherein the biologically active filtered pectin inhibits cancer cell proliferation in a cancer cell apoptosis model with an IC 50 less than 100 μg/mL.
25. The process of claim 14 , wherein the biologically active filtered pectin inhibits cancer cell proliferation in a cancer cell apoptosis model with an IC 50 less than 100 μg/mL.
26. The process of claim 1 , wherein the solution comprises 10%-20% w/w ethanol or 15%-25% w/w ethanol.
27. The process of claim 5 , wherein the second aqueous solution comprises 10%-20% w/w ethanol or 15%-25% w/w ethanol.
28. The process of claim 9 , wherein the solution comprises 10%-20% w/w ethanol or 15%-25% w/w ethanol.
29. The process of claim 14 , wherein the second aqueous solution comprises 10%-20% w/w ethanol or 15%-25% w/w ethanol.