Device and method for multiple analyte detection

1 . A method for making a test device, comprising:

providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm; (ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and (iii) a liquid solution comprising at least one analyte-specific reagent;

delivering a volume of said liquid solution into at least some of said wells, using said at least one piezoelectric dispenser, wherein said volume is no greater than about 1 microliter; and

placing a cover over said wells.

2 . The method of claim 1 , further comprising, prior to said placing, at least partially drying said liquid solution.

3 . The method of claim 2 , wherein said drying is effected, at least in part, by baking.

4 . The method of claim 2 , wherein said drying is effected, at least in part, by reduced pressure.

5 . The method of claim 1 , wherein said cover seals said wells.

6 . The method of claim 1 , wherein said cover is optically transparent.

7 . The method of claim 1 , wherein each of said wells comprises at least one dimension no greater than 0.2 mm.

8 . The method of claim 1 , wherein said substrate comprises at least 49 wells within an area no greater than 3 cm×2 cm.

9 . The method of claim 1 , wherein said at least one reagent comprises at least two oligonucleotides primers and a detectable probe.

10 . The method of claim 1 , wherein said robotic dispensing system comprises a plurality of piezoelectric dispensers.