In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.
1. A biosensor for measuring the concentration of a specific substance in a sample solution, wherein taurine is included in a previously-provided reagent layer, including electrodes comprising at least a working electrode and a counter electrode which are composed of an electric conductive substance, said electrodes being provided on an insulating support, wherein the concentration of the specific substance is measured employing said electrodes.
2. The biosensor as defined in claim 1 , wherein the reagent layer is formed on the electrodes or so that the electrodes are arranged in a diffusion area where a reagent in the reagent layer is dissolved and diffused in the sample solution, and the reagent layer includes at least an enzyme and an electron transfer agent.
3. The biosensor as defined in claim 1 , wherein the previously-provided reagent layer also includes maltitol or lactitol.
4. The biosensor as defined in claim 3 , wherein the reagent layer further includes a hydrophilic polymer.
5. The biosensor as defined in claim 1 , wherein the reagent layer further includes a hydrophilic polymer.
6. The biosensor as defined in claim 1 , wherein taurine is included in the previously-provided reagent layer so that it is dissolved in the sample solution.