Dispensing of a diagnostic liquid onto a diagnostic reagent
View Patent ↗Biological fluid samples are deposited by methods that produce a uniform layer of the sample over a reagent-containing surface. In one embodiment, a nozzle having multiple openings is used to deposit a sample over the reagent-containing surface simultaneously. In an alternative embodiment, single droplets of the sample are deposited in a pattern on the surface, preferably in a sequence of parallel lines. The reaction between the biological sample and the reagents is read from a spectrographic image of the reagent-containing surface obtained by optical methods.
1. An apparatus for dispensing a uniform layer of droplets from a sample of a biological fluid from at least 1 mm above a surface containing reagents for reacting with said reagents comprising:
(a) a nozzle for dispensing said sample of a biological fluid, said nozzle having a closed channel ending in one or more openings having diameters greater than 100 μm and up to about 1000 μm for dispensing under pressure said sample of biological fluid as droplets having diameters in the range of 0.1 to 2 mm onto a predetermined region of said reagent-containing surface;
(b) a reservoir for supplying said biological sample to said nozzle of (a);
(c) automated means for positioning said nozzle with respect to said reagent-containing surface or said surface with respect to said nozzle such that said nozzle is positioned at least 1 mm above said reagent-containing surface during dispense such that the droplets fall onto the reagent-containing surface;
(c) means for dispensing under pressure said biological sample via said nozzle openings onto predetermined portions of said reagent-containing surface; and
(d) means for reading the results of the reaction between said biological fluid and said reagents from a spectrographic image of the reagent-containing surface.
2. An apparatus of claim 1 , wherein said predetermined region of said surface is the entire reagent-containing region.
3. An apparatus of claim 1 , wherein said predetermined region of said surface is the area contacted by one droplet dispensed by said nozzle openings.
4. An apparatus of claim 1 , wherein said predetermined region of said surface is a portion of said surface containing reagents.
5. An apparatus of claim 1 , wherein said means for reading the results of the reaction between said biological fluid and said reagents includes a source of light directed onto a predetermined portion of said spectroscopic image and a detector for measuring the light returned from said portion of said spectroscopic image.
6. An apparatus of claim 1 , wherein said predetermined region of said spectroscopic image consists of the entire reagent-containing surface.
7. An apparatus of claim 1 , wherein said predetermined region of said spectroscopic image consists of the area contacted by one droplet.
8. An apparatus of claim 1 , wherein said predetermined region of said spectroscopic image consists of a portion of said reagent-containing surface.
9. An apparatus of claim 1 further comprising means for dispensing reagent liquids onto said reagent-containing surface through the one or more openings in said nozzle.
10. An Apparatus of claim 1 wherein said nozzle dispenses droplets under pressure when said nozzle is positioned from 1 to 5 mm above said reagent-containing surface.