Stable protein storage and stable nucleic acid storage in recoverable form
View Patent ↗The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage may also optionally be preserved, i.e., the absorbed biomolecule is resistant to or resists degradation.
1. A sample storage substrate comprising
an elastomeric substrate, comprising open cell foam, closed cell foam, or a combination thereof and
a biological sample absorbed by the elastomeric substrate,
wherein the elastomeric substrate is coded,
wherein at least a portion of the biological sample is recoverable from the elastomeric substrate and
wherein the biological sample absorbed by the elastomeric substrate has a higher resistance from degradation than a biological sample not absorbed by the elastomeric substrate.
2. The sample storage substrate of claim 1 , wherein the elastomeric substrate comprises a material selected from the group consisting of polyurethane, polyvinyl alcohol, polyester, and polystyrene.
3. The sample storage substrate of claim 1 , wherein the elastomeric substrate comprises a material that is reversibly compressible to at least 1/10 of the volume of the material in non-compressed state.
4. The sample storage substrate of claim 1 , wherein the elastomeric substrate comprises a material expandable to at least 2-fold the volume in a non-expanded state of the material.
5. The sample storage substrate of claim 1 , wherein the substrate is substantially free of moisture.
6. The sample storage substrate of claim 1 , wherein the biological sample is a sample of nucleic acid, polypeptide, blood, serum, plasma, biopsied cells or tissues, sputum, mucus, cerebrospinal fluid, hair, urine, stool, semen or a combination thereof.
7. The sample storage substrate of claim 1 , wherein the biological sample comprises cells, bacteria, virus, yeast, mycoplasma, or a combination thereof.
8. The sample storage substrate of claim 1 , wherein the substrate further comprises a reagent selected from the group consisting of a buffer, chelating agent, denaturing agent, detergent, reducing agent, anti-oxidant, protease inhibitor, nuclease inhibitor, anti-microbial agent, a low water uptake saccharide, or a combination thereof.
9. The sample storage substrate of claim 1 , wherein at least 30% of nucleic acid or polypeptide or both in the biological sample is recoverable from the substrate.
10. An array comprising a plurality of compartments, wherein each compartment contains an elastomeric substrate comprising open cell foam, closed cell foam, or a combination thereof, wherein the elastomeric substrate is coded and suitable for biological sample storage.
11. The array of claim 10 , wherein the elastomeric substrate comprises a material selected from the group consisting of polyurethane, polyvinyl alcohol, polyester, and polystyrene.
12. The array of claim 10 , wherein the elastomeric substrate comprises a material that is reversibly compressible to at least 1/10 of the volume of the material in non-compressed state.
13. The array of claim 10 , wherein the elastomeric substrate comprises a material expandable to at least 2-fold the volume in a non-expanded state of the material.
14. The array of claim 10 , wherein the substrate is substantially free of moisture.
15. The array of claim 10 , wherein the substrate further comprises a reagent selected from the group consisting of a buffer, chelating agent, denaturing agent, detergent, reducing agent, anti-oxidant, protease inhibitor, nuclease inhibitor, anti-microbial agent, a low water uptake saccharide, or a combination thereof.
16. The array of claim 10 , wherein the substrate further comprises a biological sample.
17. The array of claim 16 , wherein the biological sample is a sample of nucleic acid, polypeptide, blood, serum, plasma, biopsied cells or tissues, sputum, mucus, cerebrospinal fluid, hair, urine, stool, semen or a combination thereof.
18. The array of claim 16 , wherein the biological sample comprises cells, bacteria, virus, yeast, mycoplasma, or a combination thereof.
19. The array of claim 16 , wherein at least 30% of nucleic acid, polypeptide, or both of the biological sample is recoverable from the substrate.
20. A kit comprising an elastomeric substrate, an elution solution, and an instruction for using the elastomeric substrate for storing a biological sample, wherein the elastomeric substrate comprises open cell foam, closed cell foam, or a combination thereof, and is coded and suitable for biological sample storage, and wherein the elution solution is suitable to elute the biological sample from the elastomeric substrate.
21. The kit of claim 20 , wherein the elastomeric substrate comprises a material selected from the group consisting of polyurethane, polyvinyl alcohol, polyester, and polystyrene.
22. The sample storage substrate of claim 1 , wherein the elastomeric substrate has not been treated with a polyhydric compound.
23. The sample storage substrate of claim 1 , wherein the substrate contains less than 5% moisture content.
24. The array of claim 10 , wherein the elastomeric substrate has not been treated with a polyhydric compound.
25. The array of claim 10 , wherein the substrate contains less than 5% moisture content.
26. The sample storage substrate of claim 1 , wherein the biological sample absorbed by the elastomeric substrate has a higher resistance from degradation in the absence of trehalose than a biological sample not absorbed by the elastomeric substrate.
27. The array of claim 10 , wherein the elastomeric substrate is suitable for biological sample storage in the absence of trehalose.