Sensing apparatus having rotating optical assembly
View Patent ↗An apparatus for optical sensing of samples includes an optical source, an optical assembly being rotatable about an axis, a sample holder, and a detector. The optical assembly rotates, allowing the sensing apparatus to sense results from plural locations on a sample without moving the sample. Moving the sample in a linear direction while rotating the optical assembly allows sensing of an entire sample containing multiple test sites, such as a biochip. An optical assembly containing mirrors to direct light from the optical source to the sample is provided. Preferably, light enters the optical assembly along the axis of rotation. Sensing methods consistent with the invention are also described.
1. A sensing method comprising:
generating excitation light;
rotating an optical assembly in one direction to a first position;
directing the excitation light into the optical assembly along the optical assembly's rotational axis directing the excitation light out of the optical assembly along a second axis, wherein the optical assembly's rotational axis and the second axis are substantially parallel;
directing the excitation light through the optical assembly to a first location on a sample;
detecting first emitted light;
rotating the optical assembly in the one direction to a second position;
directing the excitation light through the optical assembly to a second location on the sample;
detecting second emitted light; and
again rotating the optical assembly in the one direction to the first position.
2. The sensing method of claim 1 , wherein the sample includes a biochip.
3. The sensing method of claim 1 , further comprising moving the sample.
4. A sensing method comprising:
generating first and second excitation light;
rotating an optical assembly in one direction to a first position;
directing the first and second excitation light into the optical assembly along the optical assembly's rotational axis directing the first and second excitation light out of the optical assembly along a second axis, wherein the optical assembly's rotational axis and the second axis are substantially parallel;
directing the first and second excitation light through the optical assembly to a first location on a sample;
detecting first and second emitted light;
rotating the optical assembly in the one direction to a second position;
directing the first and second excitation light through the optical assembly to a second location on the sample;
detecting third and fourth emitted light; and
again rotating the optical assembly in the one direction to the first position.
5. The sensing method of claim 4 , further comprising directing one of the first excitation light and the second excitation light to the sample at a time.
6. The sensing method of claim 3 , wherein the moving includes moving the sample in a linear direction.