Dual bead assays including covalent linkages for improved specificity and related optical analysis discs
The invention provides compositions and methods for determining whether a target agent is present in a biological sample. The invention provides a sensitive and consumer friendly system for performing biological assays. More particularly, the invention is directed to the use of a biodisc in a dual bead assay designed to identify the presence of a target molecule. The invention further provides methods to optimize the selection of a suitable solid phase for use in a dual bead assay.
1 . A method of evaluating the suitability of a solid phase as a binding surface, the method comprising:
(i) selecting a test solid phase;
(ii) binding a probe or capture agent to a first and a second sample of the test solid phase, in a first and a second binding reaction, respectively, wherein said first binding reaction comprises a cross-linking agent and said second binding reaction lacks a cross-linking agent;
(iii) determining the amount of probe or capture agent bound to the first sample of the test solid phase;
(iv) determining the amount of probe or capture agent bound to the second sample of the test solid phase; and
(v) comparing the amount of probe or capture agent bound to the first sample of the test solid phase to the amount of probe or capture agent bound to the second sample of the test solid phase, wherein if a ratio of the amount of probe or capture agent bound to the second sample of the test solid phase to the amount of probe or capture agent bound to the first sample of the test solid phase is less than a threshold value, the test solid phase is suitable as a binding surface.
2 . The method of claim 1 , wherein the threshold value is approximately 0.2.
3 . The method of claim 1 , wherein the solid phase comprises a bead.
4 . The method of claim 3 , wherein the bead comprises a magnetic bead.
5 . The method of claim 1 , wherein the solid phase comprises a biodisc surface.
6 . The method of claim 1 , wherein the probe or capture agent comprises a nucleic acid.
7 . The method of claim 6 , wherein the nucleic acid is double stranded.
8 . The method of claim 6 , wherein the probe further comprises a linker.
9 . The method of claim 8 , wherein the linker comprises at least one polyethylene glycol moiety.
10 . The method of claim 1 , wherein the probe or capture agent comprises a protein.
11 . The method of claim 10 , wherein the probe further comprises a linker.
12 . The method of claim 11 , wherein the linker comprises at least one polyethylene glycol moiety.
13 . The method of claim 1 , wherein the solid phase is attached to a biodisc.
14 . The method of claim 1 , wherein the solid phase is within but not attached to a biodisc.
15 . The method of claim 1 , wherein the cross-linking agent comprises 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
16 . A method of evaluating the suitability of a solid phase as a binding surface, the method comprising:
selecting a test solid phase;
conjugating the test solid phase with a probe or capture agent;
washing the test solid phase with a wash solution;
heat treating the the test solid phase, thereby removing non-covalently bound probe or capture agent, and;
determining the percentage of probe or capture agent bound covalently to the test solid phase, wherein if the amount of probe or capture agent bound covalently to the test solid phase is greater than a threshold value, the test solid phase is suitable as a binding surface.
17 . The method of claim 16 , wherein the threshold value is approximately 50%.
18 . The method of claim 16 , wherein the threshold value is approximately 80%.
19 . The method of claim 16 , wherein the solid phase comprises a bead.
20 . The method of claim 19 , wherein the bead comprises a magnetic bead.
21 . The method of claim 16 , wherein the solid phase comprises a biodisc surface.
22 . The method of claim 16 , wherein said conjugation is performed in the presence of a cross-linking agent.
23 . The method of claim 22 , wherein said cross-linking agent comprises 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
24 . The method of claim 16 , wherein said wash solution is a conjugate dilution buffer.