Active immunization using a siderophore receptor protein
The invention provides a vaccine for immunizing poultry and other animals against infection by a gram-negative bacteria, and a method of immunizing an animal using the vaccine. The vaccine may contain purified siderophore receptor proteins derived from a single strain or species of gram-negative bacteria or other organism, which are cross-reactive with siderophores produced by two or more strains, species or genera of gram-negative bacteria. The invention further provides a process for isolating and purifying the siderophore receptor proteins, and for preparing a vaccine containing the proteins. Also provided is a method for diagnosing gram-negative sepsis.
1. A method for immunizing an animal, the method comprising administering to an animal a composition comprising:
siderophore receptor proteins (SRPs) extracted from an outer membrane of a bacterium of family Pasteurellaceae ; wherein the SRPs have molecular weights of about 72-96 kDa;
a non-iron regulated protein obtained from an outer membrane of a bacterium of family Pasteurellaceae , wherein the non-iron regulated protein has a molecular weight of about 34-38 kDa; and
a physiologically acceptable carrier.
2. A method for immunizing an animal, the method comprising administering to an animal a composition comprising:
siderophore receptor proteins (SRPs) extracted from an outer membrane of a bacterium of family Pasteurellaceae; wherein the SRPs have molecular weights of about 72-96 kDa;
a non-iron regulated protein obtained from an outer membrane of a bacterium of family Pasteurellaceae, wherein the non-iron regulated protein has a molecular weight of about 34-38 kDa; and
a physiologically acceptable carrier, wherein the bacterium of family Pasteurellaceae is Pasteurella spp.
3. The method of claim 2 wherein the Pasteurella spp. is Pasteurella multocida , and the composition comprises at least 3 SRPs extracted from the outer membrane of the Pasteurella multocida.
4. A method for immunizing an animal, the method comprising administering to an animal a composition comprising:
siderophore receptor proteins (SRPs) extracted from an outer membrane of a bacterium of family Pasteurellaceae; wherein the SRPs have molecular weights of about 72-96 kDa;
a non-iron regulated protein obtained from an outer membrane of a bacterium of family Pasteurellaceae, wherein the non-iron regulated protein has a molecular weight of about 34-38 kDa; and
a physiologically acceptable carrier, wherein the animal is selected from poultry, domestic fowl, livestock, a horse, a companion animal, or a human.