Rabbit monoclonal antibodies to hepatitis B surface antigens and methods of using the same
View Patent ↗Reagents, methods and immunodiagnostic test kits for the accurate detection of hepatitis B virus (HBV) infection are disclosed. The methods and kits employ novel rabbit monoclonal antibodies directed against HBV surface antigens (HBsAg) with mutations in the “a” determinant region of HBsAg.
1. A hybridoma selected from Hybridoma 99S6 (ATCC Accession number PTA-6015) or Hybridoma 99S9 (ATCC Accession number PTA-6014).
2. An antibody produced by the hybridoma of claim 1 .
3. A rabbit monoclonal antibody that recognizes the same epitope as an antibody produced by hybridoma 99S6 (ATTC Accession No. PTA-6015) or 99S9 (ATCC Accession No. PTA-60 14).
4. A solid support comprising at least one rabbit monoclonal antibody according to either of claims 2 or 3 .
5. The solid support of claim 4 , wherein the support further comprises one or more additional antibodies directed against a wild-type HBsAg or an HBsAg mutant with a mutation in the “a” determinant region.
6. The solid support of claim 5 , wherein the one or more additional antibodies comprise an additional monoclonal antibody.
7. The solid support of claim 6 , wherein the one or more additional antibodies comprise a mouse monoclonal antibody.
8. The solid support of claim 4 , further comprising at least two internal controls, wherein one of the controls defines the lower detection limit for a positive result in an immunoassay using the solid support and the other control defines a highly positive result in an immunoassay using the solid support.
9. The solid support of claim 4 , wherein the solid support is a nitrocellulose strip.