Methods for identifying conditions affecting a cell state
The present invention is directed to methods for identifying agents which affect cell state. The instant invention provides rapid and efficient methods for identifying agents which affect cell state. Methods are directed toward the screening of complex combinations of agents for their ability to affect cell state. In one embodiment, cells are incubated under suitable conditions and subjected to different agents. After an appropriate amount of time, the cells are assayed to determine what, if any, characteristics they possess. Cell characteristics can be organized in a manner such that different and novel cell states can be identified.
1 . A method of identifying a cell state comprising:
providing an array of receptacles each containing cells to be investigated;
subjecting said cells in different receptacles to different agents;
waiting a pre-determined period of time before analyzing said cells;
analyzing said cells for expression of markers;
creating a spectra representing marker expression of a cell population; and
grouping said cell populations with similar spectra.
2 . The method of claim 1 , wherein said grouping is represented in a plot or graph.
3 . The method of claim 1 , wherein said cell state occurs in the presence of at least three different treatment conditions.
4 . The method of claim 3 , wherein said treatment conditions vary by the agent or agents added to the receptacles.
5 . The method of claim 1 , wherein said grouping occurs with the aid of a computer.
6 . The method of claim 1 , wherein said grouping occurs by:
calculating the Euclidean distance between the spectra of different treatment conditions; and
ordering the measures of distance of all spectra in a Tartan plot based on similarity.
7 . The method of claim 1 , further comprising the step of plotting cells with similar expression markers in profile comprising:
a first axis representing at least two markers; and
a second axis representing the number of positive cells expressing said markers.
8 . A method of identifying a cell state, comprising the steps of:
providing a cell population;
introducing a set of agents to the cell population;
detecting a set of markers; and
creating a profile.
9 . The method of claim 8 , wherein said set of agents is at least 3.
10 . The method of claim 8 , wherein said marker is expression of a particular protein.
11 . The method of claim 8 , wherein a detection marker is used for detecting a set of markers.
12 . The method of claim 1 1 , wherein said detection marker is an antibody.
13 . A method of inducing a specific cell state, comprising the steps of:
identifying a desired cell population;
creating a specific profile for said desired cell population;
creating a cell population induced with a set of agents;
identifying a profile for said cell population induced with said set of agents; and
comparing said specific profile for said desired cell population to said profile for said cell population induced with said set of agents.