Compositions for delivering nucleic acids to cells
View Patent ↗Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.
1. A process for delivering a polynucleotide to a cell comprising: associating a cationic poly cyclic imidazolinium-containing compound capable of condensing nucleic acid with the polynucleotide to form a complex and contacting the cell with the complex.
2. The process of claim 1 wherein the polynucleotide consists of DNA.
3. The process of claim 1 wherein the polynucleotide consists of RNA.
4. The process of claim 1 wherein the polynucleotide consists of an siRNA.
5. The process of claim 1 wherein the poly cyclic imidazolinium consists of a partially acylated polyethylenimine.
6. The process of claim 5 wherein the partially acylated polyethylenimine consists of an N-propionyl polyethylenimine derivative.
7. The process of claim 5 wherein the polyethylenimine consists of linear polyethylenimine.
8. The process of claim 1 wherein the poly cyclic imidazolinium consists of partially acylated polyallylamine.
9. The process of claim 1 wherein the poly cyclic imidazolinium consists of partially acylated polyvinylamine.
10. The process of claim 1 wherein the cell consists of an in vivo cell.
11. The process of claim 10 wherein the in vivo cell consists of a lung cell.
12. The process of claim 10 wherein the in vivo cell consists of a liver cell.
13. The process of claim 12 wherein the liver cell consists of a hepatocyte.
14. The process of claim 1 further comprising: associating a polyanion with the complex to form a ternary complex having a negative ζ-potential.