Use of SNPs of MCH-R for identifying genetic disorders in maintaining the normal body weight
A process for identification of a human individual's disposition for a genetic disorder in maintaining body weight is disclosed.
1 . A process for identification of a disposition for a genetic disorder in maintaining normal body weight, comprising steps a], b] and d]:
a] isolating polynucleotides from at least one cell of an individual, wherein the polynucleotides comprise an MCH-receptor gene from that individual's genome,
b] detecting the presence or absence of at least one SNP of the MCH-receptor gene, Including the non-coding regions upstream and downstream within a range of 10 Kb from beginning and end of the coding regions, in the polynucleotides from step a], wherein the SNP correlates with the genetic disorder in maintaining the normal body weight, and
d] determining the disposition for the genetic disorder in maintaining normal body weight by analysis of the results from step b].
2 . The process of claim 1 , further comprising step c]:
c] detecting the presence or absence of at least one additional SNP of the MCH-receptor gene in the polynucleotides from step a], wherein the additional SNP does not correlate with the genetic disorder in maintaining normal body weight, and
wherein step d] comprises determining the disposition for the genetic disorder in maintaining normal body weight by analysis of the results from steps b] and c].
3 . The process of claim 1 , wherein the genetic disorder in maintaining normal body weight results in phenotypic obesitas, body overweight, Anorexia nervosa, bulimia or body underweight.
4 . The process of claim 1 , wherein the polynucleotides isolated in step a] are isolated from a tissue sample removed from the individual's body.
5 . The process of claim 4 , wherein the tissue sample has been cultivated under laboratory conditions before the polynucleotides are isolated.
6 . The process of claim 4 , wherein the tissue sample comprises epithelial cells.
7 . The process of claim 1 , wherein the presence or absence of a SNP of a MCH-receptor gene is detected by determining whether a DNA or RNA molecule corresponding to the SNP hybridizes under stringent hybridization conditions to the isolated polynucleotides of step a] or by determining whether a polymerase chain reaction using the isolated polynucleotides of step a] and a pair of primers, with one primer corresponding to the SNP, results in a polymerase chain reaction product of a predicted length.
8 . The process of claim 7 , wherein the DNA molecule or one of the primers consists of a sequence according to SEQ ID NO. 1, 2, 3, 4, 5, 6, 9 or 10.
9 . The process of claim 7 , wherein the SNP of the MCH-receptor gene is SNP133073, wherein at position 100365 of NCBI Z86090 the C is replaced by a T.
10 . A diagnostic kit for identifying a disposition for a genetic disorder in maintaining normal body weight, comprising DNA or RNA probes for detection of one or several SNPs of the MCH-receptor gene.
11 . A polynucleotide of at least 12 nucleotides, comprising a portion of a MCH-receptor gene sequence wherein at position 100365 of NCBI Z86090 the C is replaced by a T (SNP133073).
12 . The polynucleotide of claim 11 , wherein the polynucleotide is at least 17 nucleotides long.