Cinnamon extract enriched for polyphenols and methods of preparing same
The present invention provides extracts enriched in total phenols and methods of producing the same. In one embodiment, the extract is prepared from a woody biomass such as cinnamon. The enriched extracts can be prepared by a novel column purification step using a polymer resin that releasably adsorbs the phenolic compounds but does not retain polar non-phenolic compounds, wherein the resin comprises aromatic rings substituted with one or more electron-withdrawing groups.
1 . A method of preparing an extract enriched in total phenols from a woody biomass known to contain phenolic compounds, said method comprising:
a) extracting said woody biomass with an extraction solvent to form a crude extract comprising proanthocyanidins, anthocyanins, and non-phenolic compounds;
b) filtering said crude extract;
c) contacting said filtered extract with a resin, wherein said resin releasably adsorbs said phenols but does not retain said non-phenolic compounds;
d) washing said resin with a wash eluent to elute said non-phenolic compounds;
e) eluting the resin with a first eluent and collecting a first fraction containing phenols;
f) eluting the resin with a second eluent and collecting a second fraction containing phenols; and
g) isolating the fraction from step e) or step f) or combining said fractions from steps e) and f) to obtain said extract enriched in total phenols, wherein said extract has substantially depleted levels of said non-phenolic compounds.
2 . The method of claim 1 , wherein said crude extract is prepared by extracting dried or fresh woody biomass with an acidified extraction solvent.
3 . The method of claim 2 , wherein said acidified extraction solvent comprises an aqueous solution having between about 0-95% ethanol and between about 0-5% acid.
4 . The method of claim 3 , wherein said acid is sulfuric acid, acetic acid or hydrochloric acid.
5 . The method of claim 2 , wherein said acidified extraction solvent comprises an aqueous solution having between about 0-100% methanol and between about 0-5% acid.
6 . The method of claim 5 , wherein said acid is sulfuric acid, acetic acid or hydrochloric acid.
7 . The method of claim 1 , wherein said enriched extract comprises between about 10-99% total phenols.
8 . The method of claim 12 , wherein said enriched extract comprises at least 12.5 % total phenols.
9 . The method of claim 12 , wherein said enriched extract comprises at least 25% total phenols.
10 . The method of claim 1 , wherein said woody biomass is Cinnamom spp.
11 . The method of claim 1 , wherein step (a) further comprises adding pectinase to said crude extract.
12 . The method of claim 1 , further comprising adding an excipient to said composition.
13 . The method of claim 12 , wherein said excipient is selected from the group consisting of preservatives, carriers, buffering agents, thickening agents, suspending agents, stabilizing agents, wetting agents, emulsifying agents, coloring agents and flavoring agents.
14 . An extract enriched in total phenols prepared by the method of claim 1 .
15 . The extract of claim 14 , wherein said woody biomass is Cinnamomum spp.
16 . The extract of claim 15 , wherein said extract has a total phenol concentration greater than 12.5%.
17 . The extract of claim 16 , further characterized by the capability of exerting an anti-diabetic and anti-lipidic effect as demonstrated in in vitro assays.
18 . The extract of claim 16 , further characterized by the capability of exerting a thermogenic effect.
19 . The extract of claim 16 , combined with one or more antimutagenic agents.
20 . The composition of claim 19 , wherein said antimutagenic agent is selected from the group consisting of green tea extracts, catechins, epicatechins, epigallocatechins, gallocatechins, and flavonoids.