Microinjection devices and methods of use
View Patent ↗The present invention provides for microinjection devices comprising a needle and a viewing instrument wherein the viewing instrument provides magnified viewing of an object to an operator from an angle other than a right angle to the object.
1. A method comprising:
viewing a germinal disc under magnification at an angle to the germinal disc of less than 90°; and
injecting a nucleic acid component to the germinal disc through a needle.
2. The method of claim 1 wherein the nucleic acid component is a vector.
3. The method of claim 2 wherein the vector is a non-viral vector.
4. The method of claim 1 wherein the germinal disc is a chicken germinal disc.
5. The method of claim 1 comprising delivering the germinal disc to the oviduct of a recipient avian female.
6. The method of claim 1 comprising inserting the needle into the germinal disc.
7. The method of claim 6 wherein inserting the needle into the germinal disc comprises penetrating a vitelline membrane.
8. The method of claim 1 comprising allowing the germinal disc to develop into a chick.
9. The method of claim 1 wherein the needle includes a bevel.
10. The method of claim 1 wherein an oscillator imparts an oscillation to the needle.
11. The method of claim 1 wherein the oscillation of the needle includes an amplitude of between about 0.001 nm and about 100 μm.
12. The method of claim 1 wherein the angle is between about 30° and about 70°.
13. The method of claim 1 wherein the viewing is through a lens.
14. The method of claim 1 wherein the viewing is through a borescope.
15. A method comprising:
viewing the surface of a genninal disc under magnification at an angle to the germinal disc of less than 90° and
injecting a substance to the germinal disc through a needle.
16. A method comprising:
viewing a germinal disc under magnification at an angle to the germinal disc of between about 1° and about 89°;
inserting a needle into the germinal disc; and
injecting a nucleic acid component into the germinal disc through the needle.
17. The method of claim 16 wherein the nucleic acid component is a vector.
18. The method of claim 17 wherein the vector is a non-viral vector.
19. The method of claim 16 wherein the germinal disc is a chicken germinal disc.
20. The method of claim 16 comprising delivering the germinal disc to the oviduct of a recipient avian female.
21. The method of claim 16 comprising inserting the needle into the germinal disc.
22. The method of claim 16 wherein inserting the needle into the germinal disc comprises penetrating a vitelline membrane.
23. The method of claim 16 comprising allowing the germinal disc to develop into a chick.
24. The method of claim 16 wherein the needle includes a bevel.
25. The method of claim 16 wherein an oscillator imparts an oscillation to the needle.
26. The method of claim 16 wherein the oscillation of the needle includes an amplitude of between about 0.001 nm and about 100 μm.
27. The method of claim 16 wherein the angle is between about 30° and about 70°.
28. The method of claim 16 wherein the viewing is through a lens.
29. The method of claim 16 wherein the viewing is through a borescope.