Method of making protein in an egg of a transgenic chicken
View Patent ↗The invention includes isolating pharmaceutical proteins from avian hard shell eggs containing pharmaceutical proteins wherein the pharmaceutical proteins are exogenous to the egg.
1. A method of producing an exogenous protein from an egg of a transgenic chicken, the method comprising:
a) obtaining a transgenic chicken comprising a nucleic acid sequence encoding the exogenous protein operably linked to a promoter, wherein the nucleic acid is derived from a replication-deficient avian leukosis virus, and wherein the exogenous protein is expressed in the tubular gland cells of the oviduct magnum, secreted into the oviduct lumen and deposited into egg white of the transgenic chicken; and
b) isolating the exogenous protein from the egg white of an egg laid by the transgenic chicken.
2. The method of claim 1 wherein the exogenous protein is selected from the group consisting of a hormone, an antibody and a cytokine.
3. The method of claim 1 wherein the exogenous protein is a cytokine.
4. The method of claim 3 wherein the cytokine is selected from the group consisting of granulocyte macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), erythropoietin and interferon.
5. The method of claim 1 wherein the exogenous protein is glycosylated.
6. The method of claim 1 wherein the exogenous protein is a human protein.
7. The method of claim 1 wherein the exogenous protein is present in an amount in a range of between about 0.1 μg per hard-shell egg and about 1 gram per hard-shell egg.
8. A method of producing a pharmaceutical protein from an egg of a transgenic chicken, the method comprising:
a) obtaining a transgenic chicken comprising a nucleic acid sequence encoding the pharmaceutical protein operably linked to a promoter, wherein the nucleic acid is derived from a replication-deficient avian leukosis virus, and wherein the pharmaceutical protein is expressed in the tubular gland cells of the oviduct magnum, secreted into the oviduct lumen and deposited into egg white of the transgenic chicken; and
b) isolating the pharmaceutical protein from the egg white of an egg laid by the transgenic chicken.
9. A method of producing exogenous cytokine from an egg of a transgenic chicken, the method comprising:
a) obtaining a transgenic chicken comprising a nucleic acid sequence encoding the exogenous cytokine operably linked to a promoter, wherein the nucleic acid is derived from a replication-deficient avian leukosis virus, and wherein the exogenous cytokine is expressed in the tubular gland cells of the oviduct magnum, secreted into the oviduct lumen and deposited into egg white of the transgenic chicken; and
b) isolating the exogenous cytokine from the egg white of an egg laid by the transgenic chicken.
10. The method of claim 9 wherein the cytokine is selected from the group consisting of granulocyte macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), erythropoietin and interferon.