Circovirus sequences associated with piglet weight loss disease (PWD)
View Patent ↗The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection is further provided. Pharmaceutical, including vaccines, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
1. A host cell transformed with vector capable of expressing at least one polypeptide encoded by a nucleic acid sequence comprising a sequence having at least 90% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25.
2. The host cell of claim 1 , wherein the vector is a baculovirus vector.
3. A host cell transformed with vector capable of expressing at least one polypeptide encoded by a nucleic acid sequence comprising a sequence having at least 95% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25.
4. The host cell of claim 3 , wherein the vector is a baculovirus vector.
5. A host cell transformed with vector capable of expressing at least one polypeptide encoded by a nucleic acid sequence comprising a sequence having at least 95% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25.
6. The host cell of claim 5 , wherein the vector is a baculovirus vector.
7. A host cell transformed with vector capable of expressing at least one polypeptide encoded by a nucleic acid sequence comprising a sequence having at least 95% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 95% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25.
8. The host cell of claim 7 , wherein the vector is a baculovirus vector.
9. A vaccine composition, comprising the transformed host cell of claim 1 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
10. A vaccine composition, comprising the transformed host cell of claim 2 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
11. A vaccine composition, comprising the transformed host cell of claim 3 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
12. A vaccine composition, comprising the transformed host cell of claim 4 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
13. A vaccine composition, comprising the transformed host cell of claim 5 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
14. A vaccine composition, comprising the transformed host cell of claim 6 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
15. A vaccine composition, comprising the transformed host cell of claim 7 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
16. A vaccine composition, comprising the transformed host cell of claim 8 and a pharmaceutically or veterinarily acceptable carrier, wherein said nucleic acid sequence encodes an immunogenic protein that induces a protective response effective against infection by a piglet weight loss disease virus.
17. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 1 and a pharmaceutically or veterinarily acceptable carrier.
18. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 2 and a pharmaceutically or veterinarily acceptable carrier.
19. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 3 and a pharmaceutically or veterinarily acceptable carrier.
20. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 4 and a pharmaceutically or veterinarily acceptable carrier.
21. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 5 and a pharmaceutically or veterinarily acceptable carrier.
22. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 6 and a pharmaceutically or veterinarily acceptable carrier.
23. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 7 and a pharmaceutically or veterinarily acceptable carrier.
24. An immunogenic composition, comprising an immunizing amount of the transformed host cell of claim 8 and a pharmaceutically or veterinarily acceptable carrier.
25. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 9 .
26. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 10 .
27. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 11 .
28. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 12 .
29. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 13 .
30. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 14 .
31. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 15 .
32. A method for treating or preventing porcine circovirus type B infection in a mammalian subject, comprising administering to said subject a therapeutically effective amount of a vaccine according to claim 16 .
33. A method of recovering protein expressed by the open reading frames of PCVB, said method including the steps a) infecting cells in growth media with a vector comprising a nucleic acid sequence having at least 90% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25; b) causing said vector to express said protein; and c) recovering said expressed protein.
34. The method of claim 33 , wherein the vector is a viral vector.
35. The method of claim 34 , wherein the vector is a baculovirus vector.
36. A method of recovering protein expressed by the open reading frames of PCVB, said method including the steps a) infecting cells in growth media with a vector comprising a nucleic acid sequence having at least 95% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25; b) causing said vector to express said protein; and c) recovering said expressed protein.
37. The method of claim 36 , wherein the vector is a viral vector.
38. The method of claim 37 , wherein the vector is a baculovirus vector.
39. A method of recovering protein expressed by ORF′2 from PCVB, said method including the steps a) infecting cells in growth media with a vector comprising a nucleic acid sequence having at least 90% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 95% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25; b) causing said vector to express said protein; and c) recovering said expressed protein.
40. The method of claim 39 , wherein the vector is a viral vector.
41. The method of claim 39 , wherein the vector is a baculovirus vector.
42. A method of recovering protein expressed by ORF′2 from PCVB, said method including the steps a) infecting cells in growth media with a vector comprising a nucleic acid sequence having at least 95% sequence identity to SEQ. ID. NO: 15, wherein said nucleic acid sequence comprises a sequence having at least 95% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25; b) causing said vector to express said protein; and c) recovering said expressed protein.
43. The method of claim 42 , wherein the vector is a viral vector.
44. The method of claim 42 , wherein the vector is a baculovirus vector.
45. A method of preparing a composition for inducing an immune response against PCVB, said method comprising the steps of: a) transferring a recombinant DNA comprising a nucleic acid sequence having at least 90% sequence identity to SEQ. ID. NO: 15 into a virus, wherein said nucleic acid sequence comprises a sequence having at least 90% sequence identity to at least one of SEQ ID NO:23 and SEQ ID NO:25; b) infecting cells with said virus, said cells being in a growth media; c) causing said virus to express the protein encoded by said nucleic acid sequence; d) recovering said recombinant protein; and e) combining said recovered protein with a suitable adjuvant or other pharmaceutically acceptable carrier or excipient.
46. The method of claim 45 , said method further including a step of obtaining said DNA from a transfer vector.
47. The method of claim 45 , said method further including a step of amplifying said nucleic acid from a strain of PCVB prior to cloning said nucleic acid into said transfer vector.